Study On The Effects And Mechanism Of The Combination Of Baicalein-Baicalin And Cisplatin On The Apoptosis Of Hela Cells

The incidence rate of cervical cancer is high in the world today,and the direct cause is definite.The treatment is mainly based on surgery,radiotherapy and chemotherapy,but the mortality rate has not decreased significantly.Cisplatin,a commonly used clinical chemical,has a significant therapeutic effect on various solid tumors,but it has serious side effects and drug resistance.Baicalein and baicalin,the active components of Scutellaria baicalensis Georgi,have no obvious toxic effect on normal cells,but have been proved to have good broad-spectrum antitumor effect.Based on this,this study aims to explore the effect and mechanism of baicalin baicalin complex combined with cisplatin on HeLa cells.In this study,HeLa cells of cervical cancer were cultured in vitro and drug intervention was carried out when they reached logarithmic phase.The experimental groups were negative control group,cisplatin group,baicalin cisplatin group,baicalin cisplatin group and baicalin baicalin complex cisplatin group.MTT method was used to detect the effect of drugs on cell proliferation;scratch method was used to detect the effect of drugs on cell migration;Hoechst 33258 method was used to observe the apoptosis of cells after drug intervention;western method was used The expression level of apoptosis related proteins was detected by blotting method,a nd the apoptosis pathway was used as the research clue to study the effect and mechanism of baicalein baicalein complex combined with cisplatin to induce apoptosis of He La cells.This study showed that baicalin,baicalin and their complexes can enhance the inhibition of proliferation and apoptosis induction of HeLa cells by cisplatin,and have a time-dependent relationship.The mechanism of action may be to reduce the expression of anti apoptotic genes and increase the expression of Pro apoptotic genes.Through the apoptotic pathway: mitochondrial pathway,endoplasmic reticulum pathway,caspase family can be activated,cascade reaction can be triggered,and finally apoptosis can be induced.This study provides experimental basis for the study of baicalin baicalin complexcombined with cisplatin in inducing apoptosis of cervical cancer cells.