Expression And Clinical Significance Of Filamentous Actin And Metalloproteinase 14 In Supraglottic Laryngeal Carcinoma

Objective:To study the expression and clinical significance of filamenous actin?F-actin?and matrix metalloproteinase-14?MMP-14?in laryngeal supraglottic carcinoma.Methods:The clinical data of 60 patients with supraglottic laryngeal cancer treated in the Department of Otolaryngology in the First Affiliated Hospital of China Medical University from March 2017 to October 2019 were collected.Immunohistochemistry was used to detect the expression of MMP-14 and F-actin in 60 cases of laryngeal carcinoma,<5mm proximal margin,?5mm clean margin.The expression and correlation in different specimens were statistically analyzed.Results:?1?the expression of MMP-14 and F-actin in laryngeal supraglottic carcinoma was not related to age and gender?P=0.542>0.05,P=0.791>0.05?.?2?Spearman rank correlation analysis was performed on the expressions of MMP-14 and F-actin in the laryngeal supraglottic carcinoma of the glottis.The results suggest that the two have a significant positive correlation?rs=0.418,P=0.001<0.05?.?3?The expressions of MMP-14 and F-actin in supraglottic carcinoma were positively expressed in laryngeal supraglottic carcinoma,<5mm proximal margin,?5mm clean margin,with significant differences?P=0.000<0.05,P=0.000<0.05?.The positive expression rate of MMP-14 and F-actin were significantly different between the laryngeal carcinoma tissue and clean resection tissue,near resection tissue and clean resection tissue?P<0.0167?.The positive expression rates of MMP-14 and F-actin were found in laryngeal carcinoma tissues and near margin tissues,and there was no statistical difference between the two groups?P=0.028>0.0167,P=0.041>0.0167?.?4?The expressions of MMP-14 and F-actin in laryngeal supraglotticc arcinoma were different between different clinical stages and pathological grades?P<0.05?.The positive expression rates of MMP-14 in T_3⁴ and T_1² were 70.2%?33/47?and 15.4%?2/13?,and the differences between the groups were significant?P=0.001<0.05?.The positive expression rates of F-actin in T_3-4 and T_1-2 were 61.7%?29/47?and 7.7%?1/13?,respectively,and the differences between groups were significant?P=0.002<0.05?.?5?The positive expression rates of MMP-14in lymph node metastasis and non-lymph node metastasis were 78.2%?18/23?and 45.9%?17/37?,and the differences between the groups were significant?P=0.014<0.05?.The positive expression rates of F-actin with and without lymph node metastasis were 73.9%?17/23?and 35.1%?13/37?,respectively,and the differences between groups were significant?P=0.003<0.05?.?6?There was a significant difference in the expression of MMP-14 among the well-differentiated?Moderately differentiated?poorly differentiated laryngeal carcinoma tissue?P=0.029<0.05?.The positive expression rate of MMP-14was significantly higher in the well-differentiated and poorly differentiated tissues?P=0.010<0.0167?.There was no significant difference in the expression rate of MMP-14between the well-differentiated and the moderately differentiated tissues?P=0.072>0.0167?.There was no significant difference in the expression rate of MMP-14 between the moderately differentiated and poorly differentiated tissues?P=0.530>0.0167?.The positive expression rates of F-actin in low,middle and high differentiation were 65%?26/40?and 20%?4/20?,which were significantly different from pathological grades?P=0.001<0.05?.There was a significant difference in the expression of F-actin among the well-differentiated?Moderately differentiated?poorly differentiated laryngeal carcinoma tissue?P=0.006<0.05?.The positive expression rate of F-actin in well-differentiated laryngeal carcinoma and poorly differentiated laryngeal carcinoma was statistically significant?P=0.003<0.0167?.The positive expression rate of F-actin between well-differentiated and moderately differentiated laryngeal carcinoma was statistically significant?P=0.006<0.0167?.There was no significant difference in the expression rate of F-actin between moderately differentiated and poorly differentiated tissues?P=0.973>0.0167?.Conclusion:MMP-14 and F-actin were highly expressed in laryngeal carcinoma,and there was a significant positive correlation between them.Both of them were highly expressed in the cases with low pathological stage and positive lymph node metastasis.Therefore,the detection of MMP-14 and F-actin can be used to distinguish the malignant degree,invasive ability and lymph node metastasis of laryngeal cancer,which is of great significance.