Effect Of Qidengmingmu Capsule On Intraocular Pressure And Research On Optic Nerve Protection In Rats Model Of Chronic Ocular Hypertension

Objective:Episcleral veins cauterization was used to establish a rat model of chronic high intraocular pressure glaucoma.The effects of ganglion cells,retinal reactive oxygen species and Caspase-3 provide a theoretical basis for clinical treatment of glaucoma.Methods:Forty-eight SD rats were randomly selected and randomly divided into a high-dose medication group,a low-dose medication group,and a positive control group and model group,12 in each group.The Tonolab tonometer was used to measure the intraocular pressure of the rat three days before the operation,as the baseline intraocular pressure,and the right eye was used as the model eye.The superior scleral vein was cauterized.On the 4th,7th,10 th,14th,17 th,21st day,the intraocular pressure was measured.The medication group was given intragastric administration of Qidengmingmu capsule suspensions with different concentrations after successful modeling(the concentration in the high-dose group was 900 mg/kg and the low-dose group was 600 mg/kg),and the positive control group was infused with acetoconazole suspension Stomach treatment(2.23mg/kg),the model group was treated with an equal amount of 0.9% sodium chloride solution,once a day for 21 consecutive days.The rats were sacrificed after gavage.The thickness of RGCs layer and full-thickness retina,the expression of ROS in the retina,the apoptosis of RGCs and the expression of Casepase-3 were measured.Results:1.On the first day of medication,there was no difference in intraocular pressure values between the groups(P>0.05).On the 7th,14 th,and 21 st days of medication,the IOP value of the model group was more than that of the high-dose group,low-dose group,and positive control group.High,the difference was statistically significant(P<0.05),the high-dose group compared to the low-dose group,the difference was statistically significant(P<0.05);Among them,the positive control group wascompared to the high-dose group on the 7th day of medication Compared with the low-dose group,the difference was not statistically significant(P>0.05).On the 14 th day of treatment,the positive control group had a difference in IOP value compared with the high-dose group(P<0.05),and no difference in the IOP value compared with the low-dose group.(P>0.05);On the 21 st day of medication,the positive control group had differences in IOP values between the high-dose group and the low-dose group(P<0.05),but the difference in IOP values from the low-dose group was smaller.Iop values of the model group were different from those of the high-dose group,the low-dose group and the positive control group at the 7th,14 th and 21 st days of treatment.Iop values of the high-dose group were different from those of the low-dose group.On the 14 th day of treatment,the iop values of the positive control group were different from those of the high-dose group,but not different from those of the low-dose group.On the 21 st day of treatment,the iop values of the positive control group were different from those of the high-dose and low-dose groups,but the iop values of the positive control group were less different from those of the low-dose group.2.The thickness of the RGCs layer in the model group was thinner than that in the blank control group,positive control group,low-dose group and high-dose group,the difference was statistically significant(P<0.05),the thickness of the RGCs layer in the high-dose group was lower than that in the low-dose group The group was thicker and the difference was statistically significant(P<0.05);the full-thickness retinal thickness of the model group was thinner than that of the blank control group,positive control group,low-dose group and high-dose group,and the difference was statistically significant(P <0.05),the thickness of the whole layer of the retina in the high-dose group was thinner than that in the low-dose group,and the difference was statistically significant(P<0.05).3.The RGCs apoptosis rate,retinal ROS content and Caspase-3 expression in the model group were higher than those in the blank control group,positive control group,high-dose group and low-dose group.0.05)RGCs apoptosis rate,retinal ROS content and Caspase-3 expression in the high-dose group were not different from those in thelow-dose group(P>0.05).Conclusion:1.The suspension of Qidengmingmu capsules in different dosage groups can reduce the intraocular pressure of chronic ocular hypertension glaucoma rats.2.Qidengmingmu capsule suspension can reduce retinal damage in chronic ocular hypertension rats,reduce RGCs apoptosis,reduce ROS content in the retina,and down-regulate the expression of Caspase-3,which can protect the optic nerve of glaucoma.