| Curcumin(Cur)is a natural polyphenol antioxidant which possesses various biological activities like wound healing,anti-infective,anticancer,and immunomodulatory activities.Hyaluronic acid grafted pullulan polymer is a biocompatible and non–toxic polymer which promote wound healing.However,this material is lack of antibacterial and antioxidant properties.In order to introduce antibacterial and antioxidant properties,curcumin grafted hyaluronic acid modified pullulan polymers(Cur-HA-SPu)by chemical conjugation was designed and prepared,and its film may be used to speed wound healing and help to fight infection efficacy.The structure of the product was confirmed by Fourier transform infrared spectroscopy(FT-IR)and hydrogen nuclear magnetic resonance spectroscopy(1H-NMR).Combined with the results of differential scanning calorimetry(DSC),total results showed that Cur-HA-SPu polymer was successfully synthesized.The content of curcumin in the Cur-HA-SPu polymer was determined by ultraviolet-visible spectrophotometry(UV-Vis).The swelling ratio test showed that the Cur-HA-SPu material had higher swelling water absorption than that of HA-SPu polymer,the material could be used to absorb quickly the wound exudate tissue fluid.The 10 mg/m L Cur-HA-SPu solution was applied to the back of the depilated rats,and no irritating phenomenon was observed,indicating that the material was safe.In addition,Cur-HA-SPu materials didn't cause hemolysis.The cytotoxicity test of Cur-HA-SPu material solution co-incubated with L929 cells showed that Cur-HA-SPu material had no obvious cytotoxicity at a certain concentration.Cell proliferation experiments showed that Cur-HA-SPu materials could promote cell proliferation and growth,indicating that it had high biosafety.The experimental results of the inhibition zone and the minimum inhibitory concentration showed that the Cur-HA-SPu had certain antibacterial ability against Escherichia coli and Staphylococcus aureus.Antioxidant activity research shows that compared with HA-SPu,Cur-HA-SPu polymers exhibited higher 1,1-Diphenyl-2-picrylhydrazyl radical(DPPH)scavenging ability.Compared with the HA-SPu group and the control group,the results of wistar rat wound repair showed that the wounds inflammation were slight and the wounds were significantly reduced on the 3rd day in the Cur-HA-SPu I~III group.Moreover,the wound healing in the Cur-HA-SPu group was well on the 21 st day,the scar was not obvious and the new born skin was similar to the surrounding normal skin.It can be seen that Cur-HA-SPu has better wound healing ability than HA-SPu.In this paper,curcumin was grafted onto hyaluronic acid-based pullulan polymer materials by esterification reaction.Cur-HA-SPu material has good biosafety,certain antibacterial and anti-oxidation ability.The material can effectively promote wound healing in rats.This paper provides preliminary experimental data support for Cur-HA-SPu films in wound healing. |
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