Study On The Auxiliary Protective Effect Of Pueraria Lobata-Radix Paeoniae Alba-Glycyrrhiza Uralensis Tablet On Alcoholic Liver Injury

Pueraria lobata-Radix Paeoniae Alba-Glycyrrhiza uralensis tablet is a health food with auxiliary protective effect on alcoholic liver injury,it was developed for people affected by alcoholic liver injury.Pueraria lobata-Radix Paeoniae Alba-Glycyrrhiza uralensis tablet are composed of Pueraria lobata,Radix Paeoniae Alba,Hovenia dulcis Thunnb and Glycyrrhiza uralensis,and are formulated according to the principle of compatibility of traditional Chinese medicine.Taking the effect on acute alcoholic liver injury in mice as the research method,the efficacy study on the compatibility of Pueraria lobata,Radix Paeoniae Alba and Glycyrrhiza uralensis was carried out to provide a basis for the dosage and efficacy of the formula.Pueraria lobata 4g,Radix Paeoniae Alba 2g,Glycyrrhiza uralensis 2g.Based on this compatibility ratio,the evaluation of subacute liver injury in mice was carried out.The content of serum TC,LDL-C and TBIL in the middle-dose group was lower than that of the model control group,and the difference was significant(P<0.05).The compatible extract has auxiliary protection function for subacute alcoholic liver injury.Based on the experimental functional literature,determine the safe and effective dosage of Hovenia dulcis Thunnb 4g.After the test materials are determined as qualified medicinal materials,the key process parameters of the preparation process of Pueraria lobata-Radix Paeoniae Alba-Glycyrrhiza uralensis tablet are screened,and the best extraction and preparation process is finally determined.The total water extraction process is to decoction with 8 times the amount of water 3 times,1h / time.The preparation process is: dry paste powder(74.29%)supplemented with microcrystalline cellulose(12.73%)and dextrin(weight supplement)after mixing for 20 minutes,add 85% ethanol solution to granulate(20 mesh sieve),dry for4 h and then granulate(24 mesh sieve),then add sodium carboxymethyl starch(3%)and magnesium stearate(1%)and mix for 5 minutes,then press the tablet(tablet weight 0.7g).The preparation process has been verified to be stable and controllable.The iconic components of Pueraria lobata-Radix Paeoniae Alba-Glycyrrhiza uralensis tablet were studied,that is the determination method of Pueraria lobata,Radix Paeoniae Alba and Glycyrrhiza uralensis were determined,and the stability of the methodwas determined by methodological investigation,which provided a reference for the study of Pueraria lobata-Radix Paeoniae Alba-Glycyrrhiza uralensis tablet quality standards.The investigation results of specificity,linearity,stability,precision,and repeatability are good,indicating that the detection methods of Puerarin,Paeoniflorin and Glycyrrhizic are reasonable and stable,and can be used as an effective method for the detection of the iconic components in Pueraria lobata-Radix Paeoniae Alba-Glycyrrhiza uralensis tablet.Acute toxicity test was used to classify Pueraria lobata-Radix Paeoniae Alba-Glycyrrhiza uralensis tablet as non-toxic.In experiment,a rat model of subacute alcoholic liver injury was established by gradually increasing the amount of alcohol.The model was established.Compared with the model group,the TC content in the serum of rats in the high-dose group of Pueraria lobata-Radix Paeoniae Alba-Glycyrrhiza uralensis tablet decreased significantly(P<0.01)and TG Decreased(P<0.05),the content of TC and TG in the serum of rats in the middle-dose group of Pueraria lobata-Radix Paeoniae Alba-Glycyrrhiza uralensis tablet(P<0.05),and decreased the content of TBIL in the serum of rats in the low-dose group(P<0.05).Compared with the model group,the degree of hepatocyte injury in the high,medium and low dose groups of Pueraria lobata-Radix Paeoniae Alba-Glycyrrhiza uralensis tablet was effectively reduced(P<0.05).It shows that Pueraria lobata-Radix Paeoniae Alba-Glycyrrhiza uralensis tablet have auxiliary protective effects on subacute alcoholic liver injury.