| Objective:To establish a rat model of type 2 diabetes mellitus(T2DM)by using high-sugar and high-fat diet combined with injection of streptozotocin(STZ),and to apply different intensity of exercise intervention during the establishment of the model to discuss the effects of exercise intervention on serum IL-6 and ?2-MG and renal TGF-? during the modeling of type 2 diabetic rats.Methods:Fifty SD male rats were randomly divided into a quiet control group(N),a diabetic control group(DM),a small-intensity exercise intervention group(LE),a moderate-intensity exercise intervention group(ME),and a high-intensity exercise intervention group(HE)5 groups of 10 each.Group N common feed,DM group was fed with high-sugar and high-fat diet,with free movement in cage;LE,ME,HE group had high-sugar and high-fat feeding,along with different levels of treadmill exercise during the period.At the end of the 8th week,fast the feeds with free water overnight for 12h.Group N was given intraperitoneal injection of citrate buffer,DM,LE,ME,HE group was intraperitoneally injected with 2%STZ solution.And on the 7th day after injection of STZ,collect the concentrations of serum IL-6 and ?2-MG in each group.taking the kidney tissues to have Western Blot test to detect the content of TGF-?1 in kidney tissue.Results:1?Serum IL-6:DM group had the highest content and LE group had the lowest content.The LE group was lower than the N group and the DM group(p<0.01),and the ME group was lower than the N group and the DM group(p<0.01).Group N was lower than the DM group but there was no significant difference,and HE was lower than the N and DM groups but there was no significant difference.2?Serum ?2-MG:HE content was the highest,LE group was the lowest,but serum?2-MG content in each group had no significant difference.The expression of TGF-?1 in the kidney of TGF-?1:LE group was the highest in each group,and the expression level of TGF-?1 in N group was the lowest,but there was no significant difference in the expression level of TGF-?1 between the groups.4?HE:Kidney structure of the N group was clear and complete,glomerulars are integrate and without obvious pathological changes;Kidney structures of DM group and LE group was unclear,glomerular was hypertrophic,basement membrane thickened,mesangial area widened.The number of glomerular cells increased,and no obvious inflammatory cells were observed;LE occasionally had tubule protein casts.The glomerular structure of the ME and HE groups was clear,the number of cells in the glomerulus decreased,and no obvious inflammatory cells were observed.The structure of the renal tubules was clear and no obvious protein casts were observed.5?PASM:The glomeruli structure of group N was clear and the basement membrane did not change significantly.In the DM and LE groups,the glomerular basement membrane was thickened and the mesangial area was significantly widened.There was no obvious thickening of the glomerular base in the ME and HE groups,and the mesangial area was slightly widened.Conclusion:1?Low-intensity exercise for 1.8 weeks can reduce serum IL-6 levels in rats during diabetes modeling(P<0.01),but the effect on renal injury factor levels and TGF-?1 expression in kidney tissues is not significant(p>0.05).).2?8 weeks of moderate-intensity exercise can reduce serum IL-6 levels in rats during diabetes modeling(P<0.01),but the effect on renal injury factor levels and TGF-?1 expression in kidney tissues is not significant(p>0.05).).3?8 weeks of high-intensity exercise can reduce the serum IL-6 level of rats during the modeling process but there is no significant difference(P>0.05),while the effect on the level of renal injury factor and TGF-?1 expression in kidney tissue is not very obvious(p>0.05). |
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