Preparation Of Mesoporous Molecular Sieve SBA-15 Surface Imprinted Polymer And Its Application In The Research Of Scutellaria Baicalensis Georgi Plasma Medicinal Chemistry

Due to the traditional Chinese medicine metabolism is low content,coexisted with complex components,and difficult to separate,the non-selective separation methods are not easy to detect and separate the real active substance.Thus,exploring for selective separation methods in the research of traditional Chinese medicine metabolism is essential.In recent years,molecular imprinting technology provides new opportunities in the applications involved in the highly selective separation of traditional Chinese medicine components.In this work,mesopocous molecular sieve technology,surface molecular imprinting method,computational simulation,and the research of traditional Chinese medicine metabolism were integrated to solve the crucial detecting problems of Scutellaria Baicalensis metabolism.Highly selective molecularly imprinted mesoporous molecular sieve polymer(SBA-15@MIP)was synthesized through the inorganic-organic hybrid method,and used as the solid-phase extraction(SPE)adsorbent to separate and detect Scutellaria Baicalensis metabolism from plasma samples.The detailed research can be categorized as following:SBA-15@MIP was prepared by using 3-Methacryloxypropyltrimethoxysilane(MPS)-functionalized SBA-15 as the supporting materials,baicalein(BAI)as the template molecular,acrylamide(AA)as the functional monomer,tetrahydrofuran/ethanol(3:2,v/v)as the polymerization solvent.Meanwhile,computational simulation was introduced to select the optimized functional monomer and polymerization solvent.The obtained SBA-15@MIP was evaluated by Fourier transform infrared(FT-IR),scanning electron microscopy(SEM),transmission electron microscopy(TEM),dynamic light scattering(DLS),X-ray energy dispersion spectroscopy(EDS),nitrogen adsorption–desorption isotherms,thermogravimetric analysis(TGA),dynamic sorption and static sorption tests.It revealed that the imprinted polymer still preserved the well-ordered one-dimensional pore structure ofSBA-15,and exhibited high binding capacity and fast kinetics for the rebinding of BAI.Finally,SBA-15@MIP was used as an adsorbent for SPE to detect BAI in plasma samples by HPLC.The average recovery of BAI in plasma was 94.4% with RSD of 2.9%.The excellent results suggested that the SBA-15@MIP can be effectively applied to selectively separate and enrich trace BAI from complex matrices.