| Purpose:By observing the Yixiao ointment influence on diabetic skin ulser mouseTumor Necrosis Factor-Alpha(TNF-?)and interleukin-6(IL-6).The aim of this study was to further explore the mechanism of the effect of Yixiao ointment on the healing ofdiabetic skin ulcer,and to provide experimental evidence for the clinical application of anodic cream in the treatment of diabetic skin ulcer.Material and method: Forty clean male mice were randomly divided into diabetic skin ulcer model group 30 and normal group 10.After successful modeling of diabetic skin ulcer group,10 mice were randomly selected as one effect cream group,10 as rehabilitation new fluid group and the other 10 as model group.The three groups were treated with a cream,a new solution of rehabilitation and saline daily topical skin ulcers for 21 days,the number of surviving mice observed continuously,living conditions and close observation of the color and size of the wound changes.At the end of the experiment,the specimens were taken and the new capillaries and hyperplastic fibroblasts were observed by HE staining.The expressions of IL-6 and TNF-? were detected by immunohistochemistry.Observed the differences among the groups,and applied statistical software SPSS17.0 analysis and processing of experimental data.Results: 1.The mice in the normal group had normal dietary drinking water,milder coats,milder temperament,good mental state,rapid reaction,and normal stool;in the 30 mice,4 experimental mice died due to excessive anesthesia.All 8 mice were found to have failed blood glucose tests,and the remaining 18 mice were randomly divided into an effect ointment group,a rehabilitation fluid group,and a model group.To ensure the consistency of the number of mice in each group,4 mice in the normal group were excluded.One effect cream group mice diet increased significantly,increased urine output,normal two,weight loss,good mental state,the wound moist,reddish color,granulation tissue gradually increased,the wound healing rate faster;Kang Fuxin liquid group of mice Diet and drinking water increased,the state of mind is acceptable,the wound surface is gradually shrinking trend;the model group mice also increased drinking water,urine output increased,but the spirit of the more sluggish,relatively rough fur,wound healing more slowly.2.HE staining showed that there were more capillaries and fibroblasts in the mice's wound surface in a monotherapy cream group,and a small number of inflammatory cells were distributed;a lot of new blood vessels,fibroblasts and inflammatory cells were scatteredly distributed in the Kangxinxin group;model group A large number of inflammatory cells are seen,showing a large area of inflammatory infiltration;normal group has no inflammatory cells,and a small number of fibroblasts can be seen.After treatment,the expression of IL-6 and TNF-? was detected in one effect ointment group by 0.212±0.025,0.193±0.019;in Kangfuxin solution group by 0.254±0.036,0.250±0.024;in model group by 0.310±0.032;0.318±0.029;normal group 0.116±0.016,0.121±0.018.The positive expression rate of IL-6 and TNF-? in Yixintao group was low,and the positive expression rates of IL-6 and TNF-? in Yuxinxin group and model group were higher(P <0.05).From the multiple analysis results,it can be concluded that the experimental group is better than the rehabilitation group,both of which are better than the model group.Conclusion: 1.A cream can effectively repair diabetic skin ulcers.2.A effect cream can reduce the inflammation reaction by inhibiting the expression levels of interleukin-6(IL-6)and tumor necrosis factor-alpha(TNF-?),which can accelerate wound healing and improve healing rate. |
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