The Effect Of Shugan Huazhi Capsule On The NF-κB Signal Pathway In The Liver Of NAFLD Rats

Purpose: To establish a rat model of non-alcoholic fatty liver disease(NAFLD)and observe the effect of Shuganhuazhi Capsule on TLR-4/NF-κB signaling pathway in rat liver tissue with TLR-4/NF-κB signaling pathway as the cut-in point To investigate the therapeutic effect and molecular mechanism of Shuganhuazhi Capsule on NAFLD model rats,and to provide a new treatment idea and experimental basis for the treatment of NAFLD by Chinese medicine.Material and method: Thirty male Sprague-Dawley rats were randomly divided into three groups: normal group,model group,and traditional Chinese medicine group,with 10 rats in each group.The normal group was fed with common feed,the model group and the Chinese medicine group were fed with high-fat diet.The three groups all had normal drinking water.The feeding environment was the same,the quietness was undisturbed,and the temperature and humidity were kept within a suitable range.After 8 weeks,the normal group and the model group were given normal saline for gavage as a control,and the Chinese medicine group was given gavage with Chinese herbs.Among them,the rats in the Chinese medicine group were converted according to the doses of adults and rats(the amount of drug needed for one day /70 Kg × animal weight × 6.3)The dose of the rats was calculated to be 0.4725 g/kg.Rats in each group were sacrificed after 4 weeks of treatment.Blood was collected from the abdominal aorta,serum was separated,liver function,serum lipids,serum inflammatory cytokines IL-6 and TNF-α levels were measured,and liver tissues were taken for pathological examination and TLR-4.Detection of Gene Expression Related to / NF-κB Signaling Pathway.Results: 1.Results of serum TG,TC,HDL-C and LDL-C in each group of rats:Compared with the normal group,the serum TG,TC,LDL-C content in the model group increased significantly,and the HDL-C content decreased significantly,and the difference was statistically significant(P<0.05);Compared with the model group,serum TG,TC,and LDL-C levels in the traditional Chinese medicine group were significantly lower,and HDL-C levels were significantly higher(P<0.05).2.The results of serum AST and ALT in each group of rats were as follows:Compared with the normal group,the serum AST and ALT levels in the model group increased significantly,and the difference was statistically significant(P<0.05);Compared with the model group,the content of serum AST and ALT in the Chinese traditional medicine group was significantly reduced,and the difference was statistically significant(P<0.05).3.Histopathological changes in the liver of each group of rats:The normal group of rats observed with the naked eye that the liver morphology was normal,the membrane was smooth and regular,and the color was reddish-brown.The results of HE staining showed that there were no fatty deposits and inflammatory cells in the liver cells,the cell cords were arranged normally,and the cell morphology was not abnormal.The rats in the model group observed with the naked eye that the liver shape did not change,the envelope was tense,the edges were blunt and thick,the surface was smooth,the liver was yellow,and there was a greasy sensation.The results of HE staining showed that the liver cell cables were disordered,and most liver cells were cytoplasmic.Fat drop vacuoles of different sizes can be seen in the cytes.And there are inflammatory cells;The observation of the liver with the naked eye in the Chinese medicine group showed that the liver looks normal,the surface is still smooth,the color is light purple-red,and there is no greasy sensation.The results of HE staining show that the liver cell morphology is generally normal,the size is the same,and the fat droplets in the liver are reduced and scattered.4.The results of serum IL-6 and TNF-α levels in each group of rats were as follows: Compared with the normal group,the serum IL-6 and TNF-α levels in the model group increased significantly,and the difference was statistically significant(P<0.05);Compared with the model group,the levels of serum IL-6 and TNF-α in the Chinese medicine group decreased significantly,and the difference was statistically significant(P 0.05).5.Expression of TLR-4 m RNA and NF-m RNA in liver tissues of each group of rats: Compared with the normal group,the expression levels of TLR-4 m RNA and NF-Bm RNA in liver tissue in the model group were significantly increased,and the difference was statistically significant(P<0.05);Compared with the model group,the expression levels of TLR-4 m RNA and NF-Bm RNA in liver tissues in the Chinese herbal group were significantly reduced,and the difference was statistically significant(P<0.05).Conclusion: 1.Shuganhuazhi Capsule can significantly improve the pathological changes of NAFLD and restore its normal physiological state.2.Shuganhuazhi Capsule can improve the fatty degeneration of NAFLD by regulating liver function AST,ALT levels and blood lipid TG,TC,HDL-C,LDL-C levels.3.Shugan Huazhang Capsule can reduce serum IL-6 and TNF-α levels,regulate the level of gene expression related to the TLR-4 / NF-B signaling pathway,and reduce the inflammatory response of NAFLD.The mechanism may be related to the TLR-4 / NF-B signal pathway.