Experimental Study On Curative Effect And Mechanism Of Capsule Ganbao On Nonalcoholic Fatty Liver Disease In Rat

Background: Nonalcoholic Fatty Liver(NAFL), i.e. Nonalcoholic Fatty Liver Disease(NAFLD).is a disease of high incidence and recidivation, up-to-date, it is pathogeny and pathology still dimness. Tradition Chinese Medicine(TCM) have some predominance on cure NAFLD,but it is short of ideal TCM preparation in clinic,so, it is important ot explore availability medicament on therapy of NAFLD.Objective: The result of prophase clinic and experiment show, Capsule Ganbao(GB) has preferably curative effect on NAFLD,for more probe into the mechanism of NAFLD and the herapy of NAFLD, accordingly,it can offer preferably theoretics and experimentation thereunder for clinic.Methods: Sixty SD rats were randomly divided into six groups: normal group, dongbaogantai(DB) group ,model group, GB lowest group, GB moderate group and GB maxim group. Rats of normal group were given common forage; the remnant rats were fed high fat diet,on the fourteenth day after mold making , GB lowest ,moderate and maxim groups were began to be administed respectively at dose of 5g/kg, l0g /kg, 20g /kg of GB mixture, DB group was given at dose of 0.54g/kg of DB mixture .After twenty-eighth days, collected blood 5ml from belly mostly artery, separate serum. The levels of Cholesterol(TC), the triglycerid(TG), Free fatty acid(FFA),the high density lipoprotein cholesterol(HDL-C),fasting blood sugar (FBS),insulin(INS), insulin sensitivity index(lSl), tumor necrosis factora(TNF-a), Leptin(LP),superoxide disutase (SOD) and malondiadehyde(MDA)were measured,immediately paunch rat liver, measure the weight of liver, calculate liver index,and investigated the issue of liver through microscope of optical. Result:GB can ameliorate remarkably fatty denaturalization of rat liver, decrease the hoist singularly index of ALT and AST, decrease high expression fatty cell factor(FFA, TNF-α, LP), alleviate insulin resist(IR), restrain the come into being of MDA,increase the active of SOD,and regulate the turbulence of fatty metabolizability. GB maxim group's effect is better than DB group.Conclusion:Insuffciency of the Spleen,Retention of Phlegm-dampness in the SpIeen,Dampness and heat in the Liver Channel,and the Stanancy of both Blood and Qi is the basic pathology mechanism.In treating,the phlegm-dampness should be removed,the spleen should be invigorated,the pathogenic of liver should be cleared away,and the blood circulation should be promoted.lt' s mechanism possibly has something with reduce expression of fatty cell factor(FFA/ TNF-α, LP), alleviate insulin resist(IR), improve liver function, restrain the come into being of MDA,increase the active of SOD,and regulate the turbulence of fatty metabolizability, etc.The prescription'collocation is in reason,it'is a availability prescription of cure NAFLD...