| Objective: The dissertation is to observe the effects of Changningfang on the general condition and pathological changes of rats with UC,as well as the m RNA content of NF-?B and the serum levels of TNF-? and IL-17,so as to explore the mechanism of changningfang in the treatment of UC.Material and methods: 50 male SPF SD rats,weighing(190±10)g and aged 6-8 weeks,were randomly divided into the model group(40 rats)and the blank control group(10 rats).In the model group,after one week of adaptive feeding,modeling used trinitrobenzene sulfonic acid-ethanol compound mold making method.After fasting for 24 h,rats in the model group were injected with tnbs-50% ethanol solution in a rubber tube 8cm into the anus at a dose of 50mg/kg.The rats in the blank group were given an enema with normal saline of equal volume.After modeling,the rats in the model group were randomly divided into the model control group,the Changningfang group,the mesalazine group and the tin powder group.There were 4 groups with 10 rats in each group.On the third day after modeling,the blank group,the model control group,the Changningfang group,the mesalazine group and the tin powder group were given normal saline,normal saline,changningfang decoction,mesalazine enema solution and tin powder.The drug was administered by enema as 2ml/time,2ml/time,6.8g/kg,6.25g/kg and 0.1g/kg respectively.The general condition of the rats was closely observed during the treatment.Seven days later,the rats were anesthetized after fasting for 24 hours and the blood was collected from the abdominal aorta.Changes in colonic mucosal tissues were observed by naked eyes and microscopy.Serum levels of IL-17 and TNF-? were detected by ELISA,and m RNA levels of NF-?B in colonic tissues were detected by RT-q PCR.Results: 1.the general conditions of rats:During modeling,rats in the model group ate and drank less,slept more and moved less.The rats had sparse and dull hair,weight loss,mucous thin stools and filth sticking around the anus,and the occult blood test was positive.During the treatment,the rats were listless,and food intake of them decreased.They had arched back,dry hair that is easy to fall off,and their weight further decreased.The rat have mucus bloody stool and OBT was positive.The DAI score of each group was higher than that of the blank group(p < 0.01).After treatment,compared with the model group,the symptoms of each drug group were improved,and the most obvious improvement was found in the Changningfang group.DAI scores of each treatment group were lower than those of the model group(P < 0.01),and the DAI scores of the Changningfang group were significantly lower than those of the western medicine group and the tin powder group(P < 0.05).CMDI scores of western medicine group and Changningfang group were lower than that of tin powder group(p < 0.05)2.Pathological manifestations:In the model group,there were colonic ulcerations,most of which reached the muscle layer or serosal layer,with visible structural damage,necrosis,edema,inflammatory infiltration and basement recess destruction in the surrounding tissues.The pathology of each treatment group had different degree of improvement.Ulceration reached submucosa or superficial muscle layer,and inflammation reached submucosa or superficial muscle layer.Irregular basement recess and abscess were observed.3.the ELISA results:The serum levels of IL-17 and TNF-? in the model group were higher than that in the blank group(p < 0.01).The serum levels of IL-17 and TNF-? in the western medicine group,the Changningfang group and the tin powder group were lower than that in the model group(p < 0.01).The serum contents of IL-17 and TNF-? in the western medicine group and the Changningfang group were lower than that in the tin powder group(p < 0.05).4.NF-?B m RNA results: The m RNA expression of NF-?B in the colon of rats in the model group was higher than that in the blank group.Compared with the model group,m RNA expression of NF-?B in the colon of rats in the western medicine group,the Changningfang group and tin powder group was lower(p < 0.01).There was no significant difference in the expression of NF-?B m RNA between the Changningfang group and the western medicine group.Conclusion: 1.Changningfang can improve the general state of UC rats,including mental state,activity,eating and drinking amount,body weight and stool status,etc.The effect of Changningtang on improving DAI score is better than the western drug mesalazine and tin powder(p < 0.05).2.Changningfang has a certain therapeutic effect on rats with UC model,the mechanism of which may be related to the decrease of serum levels of TNF-? and IL-17 in rats and the decrease of NF-?B m RNA expression in colon tissues.3.The effect of changningfang on the contents of serum TNF-? and IL-17,the expression of NF-?B m RNA in the colon,the CMDI score and the pathological lesions in rats with UC was similar to that of mesalazine. |
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