| Using sequential dilutions of the amino acid glycine, and of either ninhydrin or 1,2-indanedione as the chemical developer, the stoichiometry of the reactants will be investigated based on the color produced on filter paper. The characteristic evaluated in ninhydrin will be its Ruhemann's Purple color using visual observation, a UV-Vis Spectrophotometer, and a Xerox photocopier. For 1,2-indanedione, fluorescence will be evaluated by a fluorescence spectrophotometer, and visually by an alternate light source.; Also in this study the dilutions of the amino acid glycine and ninhydrin or 1,2-indanedione will each be mixed into one beaker and allowed to react in solution. Each of the eleven beakers will contain a known ratio of ninhydrin or 1,2-indanedione to glycine. The product being formed will then be evaluated by the same methods used above on the sequential solutions on paper. |
