| A multiplex genotyping system was applied to Atlantic salmon ( Salmo salar) being reared by the Atlantic Salmon Broodstock Development Program in Chamcook, New Brunswick. Nine microsatellite loci were used to determine the parents of 98% of 305 progeny from the 1998 year-class reared in a sea cage. The genotyping system validated family identity of 299 fish, which had been initially identified by branding. The genotyping system was also able to discriminate among all but 5 of 79 families in the 1998 year-class when progeny genotypes were generated from genotypes of known parental pairs. In a second experiment, two quantitative trait loci for body weight were identified, using progeny from two full-sib families of the 1998 year-class. Out of 25 microsatellite loci tested, association between variation and body weight was detected at two of these microsatellite loci. The QTL explained between 9.6 and 14% of the variation in body weight. |
