Preparation And Stability Of Cyanidin-3-O-Glucoside And Starch Nanoparticle Complex

Cyanidin-3-glucoside(C3G)is the most common,most widely distributed and abundant anthocyanin compounds.It has excellent antioxidant,anti-inflammatory and anti-aging abilities,and is widely used in food,pharmaceutical and other industries.However,due to the poor stability,the bioavailability of C3G is low.Starch nanoparticles(SNP)interact with C3G to form a complex to improve the instability of C3G and its bioavailability.Therefore,by determining the optimal conditions for the combination of the two,this experiment conducted structural analysis,stability and antioxidant capacity measurement,and simulated digestion of the C3G-SNPS complex in vitro,and drew the following conclusions:(1)Screening of the best combination conditions of C3G and SNPThe particle size of SNP obtained by solvent replacement method was relatively uniform,and the yield was 92.27%,higher than that by enzymatic hydrolysis method(74.6%).The SNP prepared by solvent replacement method was combined with C3G to obtain the C3G-SNPscomplex.Distilled water was determined as the reaction system,the mass ratio of C3G and SNP was 1:12,p H was 3,the binding time of 60 minutes was the best binding condition,and the highest binding rate was 84%.(2)Structural characteristics analysis of C3G-SNPs complexThe diameter of the C3G-SNPs composite was about 100 nm,and the stable morphology was observed clearly by SEM and TEM.After binding,high overlap was observed.C3G and SNP were connected by a fibrous bridge and tightly bound by the structure.Through the combination with C3G,the crystallinity of SNP has been improved to some extent.Through FTIR analysis,it is concluded that CH bond and OH bond are the main chemical bonds involved in the binding,and it is proved that SNP protects C3G after binding with C3G through non-covalent bond.(3)Study on stability and antioxidant activity of C3G-SNPs complexUnder different treatments,the C3G-SNPs composite showed higher C3G retention rate and antioxidant capacity than the C3G monomer,which fully indicates that THE SNP has an obvious protective effect on C3G.After treatment,the effect of oxidant on compound was significantly higher than that of reductant.The C3G retention rate and FRAP values in the experimental group after different concentrations of Fe³⁺treatment were significantly higher than those in the control group,which proved that SNP and Fe³⁺reaction effectively protected C3G and thus improved its retention rate and antioxidant capacity.(4)Study on simulated digestion of C3G-SNPs complex in vitroAfter digestion by saliva and stomach for 1h,no significant degradation of C3G occurred in both groups.Stomach to digest after 2h,C3G-SNPs complex total antioxidant value increased significantly,intestinal digestion,after 1h C3G-SNPs complex C3G retention rate increased by 14.03%,compared with the single C3G solution of intestinal digestion after 2 h,C3G-SNPs compounds in the solution C3G retention and antioxidant value increased significantly,in the in vitro digestion of C3G,SNP of C3G protective,improves the in vitro digestibility of C3G.These results lay a certain foundation for the future research on the interaction between C3G and starch,and also provide a theoretical basis for the research and development of related products.