| Environmental hormones are exogenous substances that can disturb the normal balance of hormones in organisms.Long-term exposure to the environmental hormones may lead to reproductive system problems,cardiovascular diseases,liver and heart system problems,etc.In the production process,it may lead to the daily necessities,food and drinks obtained from subsequent production containing environmental hormones to a level which can affect human health once the raw materials are not strictly controlled and tested.In addition,agricultural residues,industrial residues and medical wastes are also inevitable sources of environmental hormones.Therefore,rapid and sensitive detection methods need to be developed urgently.In this paper,an electrochemical sensor and a fluorescence sensor based on molecular imprinting technology were constructed to detect two common environmental hormones,nonylphenol(p-NP)and 4-nitrophenol(4-NP),respectively.Based on this,highly sensitive and specific detection methods were developed for nonylphenol and 4-nitrophenol.(1)An electrochemical sensor based on molecular imprinting technology was constructed to detect nonylphenol:Using pyrrole as the functional monomer,nonylphenol(p-NP)as the template molecule and nano-Ti O2as the carrier,nonylphenol/polypyrrole/nano-titanium dioxide molecularly imprinted polymer(p-NP-PPy@Ti O2MIP)was obtained by chemical oxidative polymerization.An electrochemical sensor(PPy@Ti O2MIP/Nafion/GCE)based on the molecularly imprinted polymer modified electrode was constructed.The sensor had good specificity and high efficiency in the adsorption of nonylphenol.The nonylphenol adsorped on the electrode had a sensitive electrochemical response.The detection method of nonylphenol based on the sensor had a wide linear detection range(1.00×10-8-8.00×10-5mol/L)and a low detection limit of(3.91×10-9mol/L).The method had high sensitivity and selectivity.It needn't rely on expensive large equipment and the pretreatment is simple.Moreover,the sensor can ensure the accuracy of the results while achieve a fast and efficient low-cost detection.(2)A fluorescence sensor based on molecular imprinting technology was constructed to detect 4-nitrophenol:A molecularly imprinted polymer with 4-NP as template and3-aminopropyl)triethoxysilane(APTES)as functional monomer was prepared on silane-functionalized carbon quantum dot(CDs)surface by inverse phase microemulsion method,and a series of conditions in synthesis and detection were optimized.The synthesized molecularly imprinted complex had a specific recognition and high adsorption performance for 4-NP.Under optimal synthesis and detection conditions,the fluorescence quenching value and the log value of the concentration of 4-NP showed good linearity in the concentration range of 1.00×10-9-1.00×10-5mol/L,and the detection limit was as low as 4.6×10-10mol/L.The method had high sensitivity and selectivity. |
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