| Soybean contains rich nutrients,and because China is a big consumer of soybean products,it will produce corresponding by-product soybean dregs.Protein and dietary fiber are the main nutrients in soybean dregs.The hydrolysates of small molecules produced by enzymatic hydrolysis of these proteins also have the functions of anti-oxidation and anti fatigue,Dietary fiber is one of the seven most important nutrients for human body,which has the function of preventing obesity and cardiovascular and cerebrovascular diseases.But these soybean dregs are not easy to store and transport,which basically cause waste of resources.The study turned waste into treasure,which not only reduced the environmental burden,but also made the protein and dietary fiber of soybean residue fully utilized.Enzyme method is very specific in the process of hydrolysis and extraction,and it does not bring pollution.Therefore,the enzyme method was used to prepare the hydrolysate peptide and insoluble dietary fiber of soybean dregs.The results showed that the extracted dietary fiber was used to prepare the milk yoghurt,which could provide some ideas for the application of the insoluble dietary fiber.The experiment was conducted with trypsin and alkaline protease as raw material.The index of the experiment was the degree of hydrolysis of soybean residue protein.The single factor experiment showed that alkaline protease was better than trypsin,and the response surface of the enzymatic hydrolysis process was optimized.The predicted value was p H 9.23,time 3.05 h,enzyme dosage 8108.11 u/g,temperature53.21?,hydrolysis degree 91.47%.According to the actual situation,the optimal extraction conditions were p H 9.25,hydrolysis time 3.00 h,enzyme dosage 8100 u/g,hydrolysis temperature 53?.The hydrolysis degree was 91.23%,and the influence of technological conditions on the hydrolysis degree was as follows:enzyme dosage>p H>time>temperature.The DPPH removal rate was the highest when the concentration of hydrolysate was 6%,reaching 71.32%.The enzymatic hydrolysis experiment was carried out with soybean dregs as raw materials,purity as index and alkaline protease.The independent variables of the response surface were determined as the amount of enzyme,p H,temperature and time.The optimum value was 93.77%of the extraction purity under the condition of temperature 50.67?,time 4.13 h,p H 9.0 and enzyme dosage 8249.54 u/g.In order to be feasible,the optimum conditions were set as follows:temperature 51?,time4.1 h,p H 9 and enzyme consumption of 8250 u/g.The results were in accordance with the single factor design conditions.The results showed that the purity of insoluble dietary fiber was 93.35%,the swelling property of the sample of insoluble dietary fiber extracted from soybean dregs by alkaline protease was 14.99 ml/g,the holding water capacity was 8.79 g/g,and the oil holding capacity was 7.96 g/g,Compared with soybean dregs,the results showed that the results showed that the yield of soybean dregs was 76.50%,92.73%,173.04%,respectively.The results showed that the insoluble dietary fiber extracted from the best technology of the above experiment was added to yoghurt,and the response surface was determined by single factor test.The response value was determined by the addition of insoluble dietary fiber,the amount of Lactobacillus inoculation,fermentation time,and the number of insoluble dietary fiber sieves as independent variables,Finally,the best formula was predicted as the amount of insoluble dietary fiber added 10.14%,the amount of Lactobacillus inoculation 3.19%,fermentation time 4.42 hours,and the number of screen number of insoluble dietary fiber 151.72,sensory score of 96.00 according to the predicted value,the actual value was 10.2%,Lactobacillus inoculation 3.2%,fermentation time 4.4 hours,The results showed that the sensory evaluation value was 95.49.The acidity of yoghurt under this condition is85 t0.The number of Lactobacillus is(10.54±1.5)×10~7cfu/m L?No E.coli was detected to meet the food hygiene standards. |
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