The Modification Of Soybean Insoluble Dietary Fiber With H₂O₂ And Its Lead Adsorption Capacity

With the development of industry,heavy metal pollution is one of the most serious environmental pollution problems in the world.Heavy metal pollutants from metallurgical,mining,electronic and other industrial production and solid waste enter the water,soil and other environment in the form of non biodegradable ions,and enter the organism with the food chain.The long-term accumulation of heavy metals in the body will damage the nervous and blood system of the human body,can cause heart,lung,kidney and bone diseases,and even cause organ canceration.A large number of studies had shown that soybean insoluble dietary fiber(SIDF)has the ability to combine with toxic heavy metals because it contains a large number of hydroxyl,carboxyl and other groups.Soybean dregs are rich in dietary fiber,which can be used as a high-quality source of dietary fiber,and can also solve the problems of environmental pollution and resource waste.In this paper,the chemical modification and structural characterization of SIDF were carried out to explore the adsorption process of modified sidf on heavy metal lead,and the preventive effect of MSIDF/SIDF on lead induced liver and kidney injury in mice was studied,which provided theoretical basis for the development and application of SIDF.1.Taking the removal rate of Pb²⁺in aqueous solution as the evaluation index,the modified SIDF was modified by H₂O₂,and the modified process was optimized by single factor test and response surface test.The results showed that:under the conditions of H₂O₂concentration of2.4%,temperature of 36?and p H value of 8.7,the removal rate of Pb²⁺in aqueous solution before and after adsorption was the highest,which was 95.05%.2.The structure of the modified and unmodified SIDF was characterized by FT-IR,XRD and SEM.The experimental results showed that the number of hydroxyl and carboxyl functional groups increases,and the crystal structure was broken,showing more porous structure,which made the adsorption capacity of MSIDF for Pb²⁺in aqueous solution enhanced.3.The adsorption process of msidf for Pb²⁺was analyzed by adsorption thermodynamics and kinetics.The results showed that the adsorption capacity of MSIDF for Pb²⁺was 142.50mg/g,the optimum p H of the solution was 5.0,and the adsorption equilibrium time was about 5min.The adsorption process of MSIDF for Pb²⁺was more consistent with the pseudo-second-order kinetic model and Langmuir model,which showed that the whole adsorption process was a monolayer adsorption process,and mainly chemical adsorption.4.To evaluate the preventive effect of MSIDF/SIDF on lead-induced liver and kidney injury by animal experiments.The results showed that MSIDF/SIDF treatment could effectively alleviate the organ swelling caused by Pb²⁺.MSIDF/SIDF preventive treatment could effectively inhibit the increase of serum ALT,AST,BUN and the decrease of MT caused by lead exposure,significantly improved the antioxidant capacity of liver and kidney in mice,and slow down the endogenous liver and kidney injury.At the same time,MSIDF/SIDF could significantly alleviate the toxic damage and morphological changes of liver and kidney caused by lead exposure in mice.In conclusion,the removal rate of Pb²⁺in aqueous solution can be effectively improved by H₂O₂modified SIDF and the adsorption process of Pb²⁺is consistent with Langmuir model and quasi second order kinetic model.At the same time,MSIDF/SIDF can effectively alleviate lead-induced liver and kidney injury in mice.Therefore,MSIDF/SIDF is an efficient and rapid adsorbent,which is expected to be used in food industry to reduce the harm of heavy metal ions to human body.