Electrochemical Chiral Recognition Of Drug Intermediates By Chiral Sensors Based On Nanomaterials

Since the configurations of different chiral drug isomers exhibit different pharmacological activities and biological behaviors,chiral recognition is becoming more and more important in the field of life sciences.Therefore,the development of effective drug intermediate enantiomer recognition technology is considered to be a serious challenge in medicine and physiology.Compared with other technologies,electrochemical technology has the advantages of high sensitivity and low environmental pollution,so it has received more attention in various drug research fields.This article mainly describes the characterization and application analysis of the prepared electrochemical sensor based on nanomaterials.1.Nanocomposite chiral interfaces(BSA-MB-MWCNTs)were prepared by using bovine serum albumin(BSA),methylene blue(MB)and multi-walled carbon nanotubes(MWCNTs),which can be used to identify the enantiomers of(1S,2S)-N,N’-dimethylcyclohexane-1,2-diamine and(1R,2R)-N,N’-dimethylcyclohexane-1,2-diamine.The characteristics of the chiral interface were observed by scanning electron microscope(SEM)and ultraviolet-visible spectroscopy(UV-Vis)techniques.Cyclic voltammetry(CV),differential pulse method(DPV)and AC impedance method are used to study the interaction between the chiral interface of nanocomposites and the enantiomers of organic intermediates.Different electrochemical response signals were obtained.2.Chitosan(CS),silver nanoparticles(Ag NPs)and multi-walled carbon nanotubes(MWCNTs)are used to modify the glassy carbon electrode to construct an electrochemical sensor.The constructed electrochemical sensor can selectively and accurately determine(R)-2-Amino-2-phenylacetamide and(S)-2-Amino-2-phenylacetamide.Through cyclic voltammetry(CV)and differential pulse voltammetry(DPV)analysis,the enantiomers showed different peak currents on the surface of the CS-Ag NPs-MWCNTs modified electrode.Through the different interactions between CS and phenylglycinamide enantiomers,the two drug intermediates can be identified by electrochemistry.3.In this paper,β-cyclodextrin(β-CD)was used as chiral selector,multi-walled carbon nanotubes(MWCNTs)were used as adsorbent,and platinum nanoparticles(PtNPs)were used as electron transfer conductor.In the process of reducing oxidized carbon nanotubes with hydrazine hydrate,β-CD were adsorbed on the multi-walled carbon nanotubes by covalent action,and β-CD-PtNPs-RCNTs were obtained.β-CD-PtNPs-RCNTs disperse well in water due to the presence of hydrophilic groups in β-CD.The electrochemical sensor designed for electrochemical recognition amino compounds(1R,2R)-(-)-2-Amino-1-(4-nitrophenyl)-1,3-propanediol and(1S,2S)-(+)-2-Amino-1-(4-nitrophenyl)-1,3-propanediol.At the same time,transmission electron microscopy(TEM)and fourier transform infrared spectroscopy(FT-IR)can be applied to characterize the surface morphology of nanomaterials,and electrochemical methods can be used to explore their repeatability and stability.