Structural Design And Error Analysis Of Biochemical Detector Based On Microfluidic Technology

The detection of biochemical indicators of the wounded/patient plays a crucial role in conditions of disaster rescue,battlefield treatment,epidemic prevention and testing in small or medium-sized medical outpatient departments.Common biochemical detectors are large in size and high in environmental conditions,so they are not suitable for disaster and wild environment.Most of the fully automated biochemical detectors are expensive and complicated,they are difficult to popularize to primary medical institutions.Therefore,the development of small size,multiparameter,high precision,easy to operate,low price,widely applicable micro emergency biochemical detector,can effectively improve the level of medical detection in China and create great social value.In response to the application requirements for rapid and accurate acquisition of biochemical indicators of injured patients in conditions of disaster rescue,battlefield treatment,epidemic protection,and testing in small or medium-sized medical outpatient departments,taking life indicators such as potassium ions,glucose,and chloride ions in the blood as the detection objects,the research on structure optimization design and error analysis of micro-miniature emergency biochemical detector based on microfluidic technology was carried out.A biochemical detection technical scheme combining centrifugal microfluidic reagent tray,LED light source and photosensitive tube detection was proposed.According to the technical scheme and the design requirements of the instrument structure,the structural optimization design of the micro-miniature emergency biochemical detector was completed.Key technologies such as development of microfluidic reagents,instrument reliability design and constant temperature control were broken through,technical difficulties such as internal optimization of the instrument layout and micro-system integration were solved.A prototype biochemical detector was successfully developed,then we analyzed the error of the prototype and proposed compensation method of each error source,the performance evaluation of each system module of the prototype and clinical comparative test of were carried out in Chong Qing Xin Qiao Hospital.The result shows that the micro-miniature emergency biochemical detector has initially reached the clinical requirements.The main work of the paper is:(1)Consulted relevant biochemical detection literature,summed up the advantages and disadvantages of the current biochemical detection instrument,and clarified the research goals and content of this article;(2)Analyzed the biochemical detection principle of the instrument and the centrifugal force of the microfluidic reagent disk.According to the design requirements of the instrument,proposed a detection technology scheme combining a centrifugal microfluidic reagent disk,LED and photosensitive tube;(3)The microfluidic reagent tray,photoelectric detection system,centrifugal separation system,constant temperature system and the stable structure of various components inside the instrument were designed,the system modules inside the instrument were rationally laid out,the instrument shell was designed,and the miniature biochemical detector Optimization design of the whole machine structure;(4)According to the structural design plan,put forward the processing requirements of each part,assemble a prototype,analyzed the influence of each error source of the instrument on the measurement result and proposed corresponding compensation measures;(5)The performance of the prototype was evaluated.Through the clinical comparative test,the instrument prototype test results were compared and analyzed.The result showed that the instrument prototype basically met the clinical use requirements.