Comparison Of Mitochondrial Gene Sequences And Hybridization Between Apolygus Lucorum And Apolygus Spinolae

Our study focused on the genetic analysis and comparison between A.lucorum in North China and A.spinolae in Xinjiang through mitochondrial genome sequencing and inter-population hybridization experiment.The main results obtained as follows.(1)Part mitochondrial genome sequences of A.spinolae populations from Manas and Aksu and A.lucorum populations from Zhanhua were sequenced and were compared to the complete mitochondrial genome of A.lucorum provided by Gen Bank.Thirteen fragments of mitochondrial genome were amplified and sequenced successfully and those sequences 9734 bases in length were aligned,revealing 60 variable sites(containing 38 parsimony informative sites and 22 singleton variable sites).The nucleotide polymorphism of the eight haplotypes is 0.00245.The NJ tree results indicated that these two species cannot be distinguished by the part of mitochondrial genome sequences.It turned out that the three mirid populations have a basically coherent genetic background.(2)In the inter-population hybridization experiment,reciprocal hybridization experiment were conducted between A.spinolae in Xinjiang(Manas,Aksu)and A.lucorum in Zhanhua,respectively.Then the F1 generation of reciprocal hybridization was used backcrossed with A.lucorum in Zhuanhua.This result shows that both hybridization generations can reproduce fertile offsprings,which means that there is no reproductive isolation between A.spinolae in Xinjiang(Manas,Aksu)and A.lucorum in Zhanhua.Based on the above results,we identified that A.spinolae and A.lucorum have a basically coherent genetic background and no reproductive isolation.This results also provide an important scientific basis for the monitoring and management of this species.