| The parthenocarpy of eggplant(Solanum melongena L.),a desirable trait in edible fruit with hard seeds,could overcome the drop of flower and fruit caused by unfavorable environmental conditions,and enhance the fruit-setting ability,increase the yield.besides,it can also improve the quality of the fruit and reduce the cost of cultivation.It was demonstrated that SmMsrA can positively promote the development of parthenocarpic fruit,but there is no difference in the CDS of SmMsrA between parthenocarpic and nonparthenocarpic materials,which is speculated to be caused by the difference in it’s promoter.In this study,a SmMsrA promoter was cloned by parthenocarpic(D-10)and a non-parthenocarpic eggplant line(03-2),and the structural characteristics and functional domains was analyzed by online tools;the interaction protein was obtained by Yeast-One hybridization experiment,and the functions of parthenocarpy related genes was analysised.At the same time,a transcriptome was used to analysis the differentially expressed genes in different fruit to find more gene related to parthenocarpic fruit,and the research results will lays a foundation for the analysis of the regulatory mechanism of parthenocarpic eggplant line D-10.The main conclusions were as follows:1.The 2433 bp promoter of SmMsrA was cloned,and there was no difference between D-10 and 03-2.9 P(N)::GUS expression vectors with deletion of 5’ end were constructed,the GUS histochemical staining show that different lengths of SmMsrA promoter could induce the expression of GUS gene,and the effect becomes stronger as the length of the promoter sequence increases,but it was clear thet the expression of GUS increasing slowly as the sequence lenger than 2002 bp,so it was speculates that 2002 bp is the full length of SmMsrA promoter.2.A yeast one-hybrid library was constructed by ovary of D-10 and 03-2 at different developmental stages.MEJA,ABA,and LTR is the main cis-elementers of SmMsrA promoter,was used as bait to screened in the library.A total of 46 interacting proteins were obtained.Gene Ontology enrichment analysis found that these proteins are involved in " Metabolic process"," cellular process"," binding" and " catalytic activity",Among which three proteins may be related to parthenocarpy,namely LTR-24(homologous with a MYB transcription factor KAN),LTR-10(homologous with a receptor kinase CKI1 protein involved in the cytokinin metabolism pathway),LTR-4(homologous with an F-box protein).These results indicate that SmMsrA gene may induce the production of parthenocarpy eggplant by mediating auxins,cytokinins and polyamines.3.Transcriptome was used to analysis the differentially expressed genes in ovary of D-10 and 03-2 on the day of anthesis,2 days and 4 days after anthesis.Eight genes might be relate d to the parthenocarpy of eggplant have been discovered,among which two genes are related t o flower development,namely Sme2.5＿04901.1＿g00003.1(homologous with LRR elongation prote in)and Novel02072(homologous with MZF2 genes specifically expressed by pollen);four genes related to the reponses of abiotic stresses,namely Sme2.5＿01830.1＿g00008.1(a MYB type transc ription factor),Sme2.5＿11537.1＿g00001.1(homologous to LRR-PLK type protein kinase PXL1),S me2.5＿06927.1＿g00005.1(homologous to a WRKY type transcription factor RID)and Sme2.5＿05426.1＿g00001.1(homologous to b HLH96);a ARF5 homologous gene Sme2.5＿00003.1＿g00017.1,and an F-box protein family homologous gene Sme2.5＿10887.1＿g00002.1. |
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