Establishment Of Seedling Cultivation System For Potato Ture Seeds And Optimization System For Virus-free Culture Conditions At Stem Tip

In order to increase the seed germination rate and survival rate of potato true seeds,the seeds of the potato varieties Zaodabai and Helan14 were used as test materials.Research was conducted on seed germination,transplanting,and mini-tubers with potato,and the seedling cultivation system for potato seeds was summarized.In order to optimize the detoxification culture system of potato stem tips,Chengdezaohe and Chengde3X were used as test materials,the techniques of potato stem tip detoxification were studied and optimized from the aspects of heat treatment,apical culture conditions,and miniature potato tubers.This study contains the following results:1.The suitable potato true seed disinfection method was 75%ethanol disinfection for 30 seconds and 1%sodium hypochlorite disinfection for 20 minutes.Zaodabai seeds were soaked in sterile water,65 rotations per minute,shake culture,culture temperature is set to 23-25°C,7 days after the germination of the seeds in the clean bench under the MS medium transfer,the germination effect of this method was increased by 24.55%compared to the control（double filter paper）.The seed germination of the Helan 14 seeds was best in the MS medium compared to the control（double filter paper pregermination）increased by 15.25%.The suitable transplanting substrate is peat soil:vermiculite:perlite（volume ratio 3:1:1）,which can only be used after high temperature sterilization of the transplanted substrate.Before transplanting,the tissue culture seedlings were opened and covered for 2 days.After transplanting,the seedlings were moistened by mulching film,and new leaves were grown to remove the film.The formula of nutrient solution suitable for mini-tubers was 826 mg/L calcium nitrate tetrahydrate,280 mg/L ammonium nitrate,786 mg/L potassium nitrate,340 mg/L potassium dihydrogen phosphate,and 493 mg/L magnesium sulfate heptahydrate.The remaining trace element and iron salt formulations,as same as Hoagland’s nutrient solution.Compared with the control（Hoagland’s nutrient solution）,the number of tubers increased by 28.71%and 31.15%.2.The tissue culture seedlings were pretreated with warm air（39°C6h+23°C18h）,which helped to increase the survival rate and detoxification rate of detoxification at the stem tip;Suitable shoot tip callus formation medium was MS+6-BA 0.15mg/L+NAA 0.1 mg/L+GA₃0.1 mg/L;The hormone concentrations in the bud induction medium of the two varieties were not the same.The optimum 6-BA concentration in shoot induction medium of chengdezaohe was 0.45 mg/L and in shoot induction medium of chengde3X was 0.15 mg/L.Adventitious buds were transferred to MS medium and seedlings were grown,then Induced in vitro microtuber,the medium was MS（7%sucrose）+B₉15 mg/L+NAA 0.3 mg/L,light intensity 1000lx,photoperiod of 12h/d.This condition helped to induce the microtubules.