Preparation And Application Of Fermentation Feed By Complex Probiotics

With the standardized development of animal husbandry and the improvement of breeding level,it is urgent to find safe,pollution-free,drug-free feed that can improve immunity and prevent diseases.It was found that probiotics had a good effect in promoting animal growth and preventing diseases.Using microorganism to produce feed and developing green antibiotics free feed have become the focus of animal nutrition research.In recent years,great progress has been made in biological feed research,especially in animal husbandry.Three probiotics,Lactobacillus plantarum R9-1,Bacillus subtilis mut-301 and Saccharomyces cerevisiae S.C,were isolated and preserved in the laboratory.Orthogonal test was used to optimize the proportion of fermentation strains,and the best inoculation combination was determined by comprehensive scoring method.The results showed that the best ratio of probiotics after fermentation was Lactobacillus plantarum r9-1: Bacillus subtilis mut-301: Saccharomyces cerevisiae S.C = 3:2:2.Furthermore,the orthogonal experiment design was used to optimize the fermentation conditions,and the comprehensive scoring method was used to find the optimal fermentation time,temperature,material water ratio and inoculation amount.The results showed that the optimum fermentation conditions were:3%inoculation quantity,3 days fermentation time,32℃ fermentation temperature and 1:0.8feed water ratio.After the optimization of feed technology,the effect of digestive enzymes on feed and different drying temperature on several digestive enzymes were investigated after fermentation.The results showed that the activities of protease activity 41.11U/g,lipase activity 0.25U/g,cellulose activity 3.92U/g,amylase activity 39.47U/g and phytase activity15.45U/g in the fermented feed were significantly higher than those in the original feed,and the enzyme activities of the five digestive enzymes could be maintained above 75% when the drying temperature was below 55 ℃.In order to test the actual effect of fermented feed,the feeding experiment of broiler was carried out.One month old broilers were fed in groups.The experimental group added 10%fermented feed to the basic feed and the control group added unfermented basic feed.The body weight was measured every 10 days.At the end of the experiment,the total antioxidant capacity of liver,the content of catalase and malondialdehyde,and the number of Lactobacillus and coliform in intestinal contents were measured.The results showed that the average daily gain of the experimental group was 24.46% higher than that of the control group,the feed meat ratio of the experimental group was 18.31% lower than that of the control group,the total antioxidant capacity of the experimental group was 54.4% higher than that of the control group,the catalase content of the experimental group was 105.98% higher than that of the control group,and the malondialdehyde content of the experimental group was 15.75% lower than that of the control group.The quantity of coliform and the content of lactic acid bacteria in broiler intestine decreased obviously,which was beneficial to maintain the intestinal balance.This research not only provides experimental and theoretical basis for the development of new fermented feed,but also provides reference for farmers or industrial production of fermented feed in the future as well as,research direction for the production of disease resistant green feed.Through the preliminary evaluation of broiler feeding test results,it shows that probiotic fermentation feed can promote the growth of broiler,and can improve the body’s antioxidant capacity,immunity and disease resistance,which has research value and certain economic benefits.