Study On The Nutritional Requirement Of Cipangopaludina Cahayensis And The Effect Of Fermented Feed On Its Intestinal Flora

Cipangopaludina cahayensisl,commonly known as the mudsnail,its meat tastes delicious,rich in nutrients,but also has health care functions,is popular among consumers,the market demand is high,the rapid development of artificial farming has become one of the important species of aquatic products in China's paddy field aquaculture.However,the nutritional study of mudsnai is weak,and there is very little research on the nutritional requirements of mudsnail,and the development and utilization of feed is in its initial stage.In order to study the nutritional requirements of mudsnail and the impact of fermented feed on the intestinal flora of mudsnail this paper takes Cipangopaludina cahayensisl as the research object,the nutritional components of the meat of Cipangopaludina cahayensis are analyzed,the suitable requirements of protein,fat,calcium and phosphorus in the compound feed of Cipangopaludina cahayensis are obtained,the influence of fermented feed on intestinal flora of mudsnail is discussed,and the basic data are provided for the research of mudsnail feed development and breeding production.The results of the study are as follows:1.Comparative analysis of nutritional components of Cipangopaludina cahayensis and other snails.The crude protein(CP),crude fat(EE),crude Ash(Ash)and amino acid(AA)content of the muscles of Cipangopaludina cahayensis,Cipangopaludina chinensis,Bellamya aeruginosa and Bellamya purificata(Heude)were determined by conventional analysis methods.The results showed that CP of Cipangopaludina cahayensis,Bellamya aeruginosa,Cipangopaludina chinensis,and Bellamya purificata(Heude)were 13.11%,10.24%,11.66%,10.27%,EE was 0.39%,0.38%,0.52%,1.05%,ash4.39%,4.54%,4.33%,4.60%,respectively.2.Effect of different protein level feeds on the production performance and survival rate of Cipangopaludina cahayensis.The mudsnails were selected from 2100 individuals weighting 7-8 g and were randomly divided into 7 groups using a single-factor test design,with 3 replicates in replicates in each group and 100 individuals in each replicate,and fed six diets of CP 15%,18%,21%,24%,27%and 30%,respectively,with no feeding in the control group,for a 60-day paddy feeding trial.The results showed that the survival rate of mudsnails fed with different protein levels was above 80%and the different between the groups was not significant.The weight gain of Cipangopaludina cahayensis was the best in the CP 24%group,which was 26.76%(P<0.05)?22.47%?24.57%?1.04%and 1.06%higher than the CP 15%,CP18%,CP21%,CP27%and CP 30%groups,respectively,but the difference between the groups was not significant.The regression equation and parabolic equations established with the mean weight gain(y)and feed protein content(x)of Cipangopaludina cahayensis were y=3.0686 x+1.2285 and y=-18.002 x~2+11.21 x+0.3558,respectively,with CP requirements ranging from 17.46%to 27.76%and an appropriate CP requirement of 24%.3.Study on the appropriate amount of protein,fat,calcium and phosphorus in the diets of Cipangopaludina cahayensis.2100 mudsnails weighting 6-7 g were randomly divided into 7 groups with 3 replicates in each group and 100 ineach replicate by uniform design test.The control group was fed with 6 kinds of compound feeds with different protein,fat, calcium and phosphorus levels,while the control group was not fed with the ompound feed,and a 60-day paddy field feeding test was conducted.The results showed that the mudsnails grew fastest in the diet group fed with CP 15%,EE 4.5%,Ca 8.5%and P 1.0%.Regression model results showed that the maximum weight gain conditiongs were 15%protein,8.5%fat,8.5%calcium and 1.0%phosphorus.4.Study on the effect of fermented feed on the intestinal flora of Cipangopaludina cahayensis.Sixty mudsnails weighing 25g were selected and randomly divided into two groups,each with 3 replicates of 10 mudsnail each.After 20 days feeding of unfermented and fermented feed respectively,mudsnail intestinal flora diversity was detected by 16s DNA high-throughput sequencing.The results showed that,compared with the nfermented feed group,the richness of the Firmicutes in the fermented feed group was increased,and the OTU,Chao 1 index and Simpson index were increased,but the ifference between the two groups was not significant(P>0.05).