| Oil peony is an emerging woody oil crop that integrates ornamental,medicinal,and oil value.It has the characteristics of high output,high oil content,high quality,and low cost.The largest planting area in the country is 1 million mu in Shandong.In Tianjin,the peony base in Tianjin Jixian County has also grown 20,000 mu.Tianjin oil peony varieties are severely degraded,and have great quality differences.In order to renew and rejuvenate the oil peony varieties in this area,select high-yield and high-quality varieties.Relying on the national color Tianxiang peony base of Dahengshang Village,Jizhou District,Tianjin,50 mu of five-year-old ’Paeonia Ostii’ peony plants were screened and screened through six indicators of flowering period（number of flowers per plant,flower diameter,etc.）The determination of the initial selection of 200 candidate plants.Then,multiple selections were conducted,and 150 candidate plants were selected by measuring 12 indicators of fruiting period（number of fruits per plant,diameter of pods,etc.）and 12 indicators of seed quality（yield per plant,oil yield,etc.）.Calculate the comprehensive score of candidate plants,and finally select the 7 plants with the highest score as excellent single plants,and analyze their differences in growth and development,yield,quality,morphology,DNA polymorphism,etc.The main research The results are as follows:1.Primary selection: Among the 200 candidate plants selected at the beginning of the flowering period,the variation range of flowering number per plant is 5-8,the variation range of flower diameter is 10.25-17.25 cm,and the variation range of carpel number is 5-9.The change range of new shoot length in flowering period is 25.75 ～ 61.00 cm,the change range of new shoot number is 4 ～ 8,and the change range of stem thickness in flowering period is 4 ～8mm.2.Re-election: Among the 150 candidate plants selected in the fruiting period,the variation range of the number of individual plants is 5-7,the variation range of the diameter of the pods is 14.13-24 mm,and the variation range of the number of anemone per plant is 23-32 The plant height varies from 42 to 110 cm,the fruit thickness varies from 6.86 to 14.00 mm,the relative increase in stem thickness varies from 0.48 to 7.00 mm,and the new shoot length varies from 37.5 to 68 cm.3.The seed quality of the 150 candidate plants selected was found,and it was found that the yield of each plant varied from 43.3 to 97 g,the oil yield rate varied from 19.58% to26.67%,and the number of seeds per plant varied from 57 to 129.The change range of the number of full seeds is 52 ～ 125,the change range of fresh weight of fruit pods is 61.6 ～ 152 g,the change range of dry weight of fruit pods is 52.7 ～ 130 g,and the change range of yield per mu is 69.3 ～ 155.2kg.4.Principal component analysis results show that the comprehensive score range of 150 candidate plants is-0.9 ～ 2.2 points.Among them,Plant No.189 had the highest comprehensive score of 2.19 points;Plant No.83 had the lowest comprehensive score of-0.87 points.According to the score,150 plants were divided into three categories: excellent,medium and poor,with a score of> 2 divided into excellent single plants,a total of 7 plants;a score between 0 and 2 was divided into medium,a total of 50 plants;score < 0 points are poor,with a total of 93 strains.The seven plants with the highest comprehensive scores are excellent single plants,which are No.4,No.42,No.73,No.105,No.141,No.153,No.189 respectively.5.Comparison of growth indexes of excellent single plants: The length of new shoots of excellent single plants is 57-61 cm,which is significantly higher than the average of 36.45 cm,which is 1.6 times the average;the number of new shoots of excellent single plants is 7-8,which is significantly higher than The average value is 5,which is 1.4 to 1.6 times the average value;the length of the new shoots of the excellent single plant is 62-68 cm,which is significantly higher than the average value of 42.53 cm,which is 1.5 times the average;the stem diameter of the excellent single plant is 12 ～ 14 mm,which is significantly higher than the average value of 8.21 mm,which is 1.5 times the average value;the relative growth of the stem diameter of the excellent single plant is 4 ～ 6mm,which is significantly higher than the average value of 2.31 mm,which is 1.7 ～ 2.6 times the average value.Among them,the growth indexes of excellent single plants No.4,No.73,No.105,No.189 were significantly higher than No.42,No.141,No.153.6.Comparison of excellent individual plant development indicators: the flowering number of excellent individual plants is 7-8,which is significantly higher than the average of5,which is 1.4-1.6 times of the average;the diameter of excellent individual plants is16.5-17.75 cm,which is significantly higher than the average The value is 13.15 cm,which is1.3 times the average value;the number of carpels per plant is 8-9,significantly higher than the average value 6,which is 1.3-1.5 times the average value;the development index of excellent single plant No.189 is significantly higher Other plants.7.Comparison of the yield index of excellent single plants: the yield of excellent single plants is 88 ～ 97 g,which is significantly higher than the average value of 56.5g,which is 1.6times the average value;the number of excellent single plant results is 6 ～ 7,which is significantly higher than the average value of 5,is 1.2 ～ 1.4 times of the average value;the diameter of the excellent single plant pod is 23 ～ 24 mm,which is significantly higher than the average value of 15.8mm,which is 1.5 times the average value;the fresh weight of the excellent single plant pod is 145 ～ 152 g,which is significantly higher than The average value is 86.2g,which is 1.7 times the average value;the number of seeds per plant per plant is 119 ～129,which is significantly higher than the average value of 75,which is 1.6 times the average value.Among them,the yield index of excellent single plants No.42,No.141,No.153,No.189 was significantly higher than No.4,No.73,No.105.8.Comparison of excellent individual plant quality indicators: the oil yield of excellent individual plants is 25.83% ～ 26.67%,which is significantly higher than the average value of22.16%,which is 1.2 times the average value.Among them,the quality of excellent single plant No.189 was significantly higher than that of No.4 and No.42.9.The comprehensive analysis results of the membership function show that: 7 excellent individual plants can be divided into three categories: high-yielding excellent individual plants are No.42,141,153,189;high-quality excellent individual plants are No.189;vigorous growth The excellent single plants are No.4,No.73,No.105,No.189.From the overall evaluation of the membership function,the best single plant is No.189,the second category is No.42,No.141,and 153,and the third category is No.4,No.73,and No.105.10.Polymorphism analysis showed that after 11 pairs of primers were used to PCR amplify excellent single plants,6 pairs of primers had good amplification effect,and a total of187 bands were amplified,with 171 polymorphic bands.The morphological ratio is 91.44%.The results show that,on the 4th,12 bands were added after the amplification compared with the control,and 7 bands disappeared;on the 42 nd,14 bands were added after the amplification compared with the control,and 8 bands disappeared;Added 10 bands and disappeared 3 bands;No.105 added 8 bands after amplification compared to the control and disappeared 6 bands;No.141 added 11 bands after amplification compared to the control and disappeared 5 bands Bands;No.153 adds 10 bands to the control after amplification and disappears 5 bands;No.189 adds 14 bands to the control after amplification and disappears 5bands.The range of genetic correlation coefficient of excellent single plant is 0.21 ～ 0.80.Among them,No.4,No.73 and No.105 are close to each other,No.42,No.141 and No.153 are close to each other,No.189 is in the middle,but the relationship with each plant has reached more than 0.61.11.The indicator contribution rate shows that when the cumulative contribution rate of the indicator reaches 60%,the main impact indicators are the yield per plant,the number of results per plant,the number of seeds per plant,the diameter of the pod,the fresh weight of the pod,and the dry weight of the pod 1.Oil yield,oil peony selection can refer to the above indicators. |
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