| Background:Severe traumatic brain injury is one of the main causes of death and disability of young people in the world.Severe traumatic brain injury often leads to the loss of nerve function,but the effect of traditional treatment methods is often unsatisfactory Therefore,how to improve the prognosis of severe traumatic brain injury has always been a difficult problem in the medical field.Some related studies have confirmed that after severe traumatic brain injury,some newborn nerve cells are produced in the injured brain tissue,but the number is extremely limited,which can not fully improve the damaged nerve function.Neurofactors,as a kind of protein which can nourish and regulate the survival of nerve cells,have been proved by many studies to promote the proliferation and anti-apoptosis of nerve cells in vitro to improve the damaged nerve function,and have been widely used in the research of neurodegenerative diseases and ischemic diseases.In the treatment of brain injury,it is rare to study the influence of nerve factors on nerve cells of rats after severe traumatic brain injury,and it is even rarer to observe the influence of nerve growth factor and basic fibroblast growth factor on nerve cells and nerve function of rats after severe traumatic brain injury.Objective:To explore the effect of different nerve factors on nerve cells in rats with severe brain injury,observe the difference of limb function recovery in different experimental groups,and explore the appropriate strategy and mechanism of exogenous nerve factors in the treatment of severe brain injury Methods: 48 SD rats were randomly divided into four groups: nerve growth factor group,basic fibroblast growth factor group,combined nerve growth factor and basic fibroblast growth factor group and control group.The model of brain injury was made according to the modified Feeney method.One day after modeling,the control group was injected with normal saline via intraventricular injection,and the experimental groups were injected with corresponding nerve factors via intraventricular injection.Immunohistochemistry and immunofluorescence were used to compare the expression of positive cells in the injured cortex,subependymal area,dentate gyrus of hippocampus and the corresponding area on the opposite side;forelimb placement test and balance test were used to observe the limb function of rats,and the difference of limb function recovery of rats in each group was compared.Results:(1)The number of neurons and glial cells in nerve growth factor group,basic fibroblast growth factor group and combined group was significantly higher than that in control group after 3 d,7d and 14d(P < 0.05),while the number of neurons and glial cells in nerve growth factor group,basic fibroblast growth factor group was significantly less than that in combined group(P <0.05),and there was no significant difference in the number of positive cells in each region between nerve growth factor group and basic fibroblast growth factor group;(2)After 7 days of modeling,the scores of nerve growth factor group,basic fibroblast growth factor group and combined group were lower than those of control group(P < 0.05),and the scores of combined group were lower than those of nerve growth factor group and basic fibroblast growth factor group(P < 0.05).Conclusion:Combined application of exogenous nerve growth factor and basic fibroblast growth factor can promote the proliferation of nerve cells and the recovery of limb function in rats. |
