Study Of Chemical Consitituents Of Potentilla Longifolia

Objective:To investigate the chemical consitituents of Potentilla longifolia.Methods:The crude extracts were obtained by using 95%ethanol to extract the reflux from the aerial parts of Potentilla longifolia,and using H2O to extract the root of Potentilla longifolia.The crude extracts were purified by a variety of separation purification methods(Sephadex LH-20,ODS,MPLC,HPLC and glycemic gel column chromatography)respectively.And the structures of compounds were identified by various nuclear magnetic means(1H-NMR,13C-NMR).Conclusion:14 compounds were isolated from the 95%ethanol extract from aerial parts of Potentilla longifolia and the root of Potentilla longifolia.On the basic of spectroscopic analysis and physicochemical properies,their structures were identified as Pernambucone(1),Orobanone(2),1-(2-methoxy-4,6-dihydroxyphenyl)-3-methyl-1-butanone(3),5,7-dihydroxy-3,8,4’-trimethoxy-flavone(4),Ursolic-acid(5),4’,5,6,7-tetrahydroxy-dihydro-flavone(6),ent-Kaurane-16β,19-dihydroxy(7),2(R)-Methyl-1-(2,4-dihydroxy-3-methyl-6-methoxyphenyl)-1-butanone(8),Phthalic acid-double(2,ethyloctyl)ester(9),Quercetin-3-O-β-D-glucoside(10),4-methoxyphenyl3-methyl-butanone-2-O-β-D-glucoside(11),(6R,9R)-3-oxo-α-ionol-9-O-β-D-glucopyra-noside(12),(+)groucin-4-O-β-D-glucoside(13),2-O-β-D-glucopyranoside-5-methoxybenzoic acid methyl ester(14),compoundsl-4,6-8,11-14 were separated from the Rosaceae plant for the first time,compounds 1-4,6-8,11-14 were isolated from Potentilla longifolia for the first time.compounds 1-4,6-8 were isolated from the aerial parts of Potentilla longifolia for the first time.Compounds 11-14 were isolated from the root of Potentilla longifolia for the first time.