Expression Of Akt And GSK3β In Myocardial Ischemia Rats And Intervention Of Irbesartan

ObjectiveExplore the pathological changes of myocardial cells in rats with myocardial ischemia in different periods;and the expression of Akt and GSK3β,and the effect of irbesartan on their expression.MethodModeling and grouping 90 adult male SD rats were randomly divided into sham-operation group(sham-operation group),myocardial ischemia group(MI group),myocardial ischemia + irbesartan treatment group(MI+IRB group),30 per group.The Sham group was only threaded and the left anterior descending coronary artery(LAD)was not ligated;the MI group was ligated LAD to make a model of myocardial ischemia;the MI+IRB group was given irbesartan after the modeling was completed,Were executed immediately,4 weeks,and 8 weeks later and collected materials.The HE staining method was used to observe the pathomorphological changes of myocardial tissue in each group at different times;Real-time fluorescent quantitative PCR(RT-PCR)was used to detect the expression levels of Akt and GSK3β mRNA in rat myocardial tissues at different moments;Western Blot was used to detect Akt,phosphorylated Akt(p-Akt)and GSK3β,The expression level of phosphorylated GSK3β(p-GSK3β)protein.The measurement data is expressed by the mean ± standard deviation(±),and the t test and one-way analysis of variance are used to compare whether the changes of the above indicators between and within each group are statistically significant(P<0.05 means that the difference is statistically significant)).Result1.HE stainingCompared with the Sham group,the rats in the MI group took samples from immediately after modeling to the 4th and 8th weeks.The damage of cardiomyocytes gradually increased,the cardiomyocytes swelled and ruptured,the nuclei were stained unevenly,interstitial edema,and inflammatory cells Infiltration and obvious necrosis were accompanied by contractile bands;the myocardial tissue changes of the specimens collected immediately after modeling in the MI + IRB group were the same as those in the MI group.In the 4th week,myocardial cells were swollen,interstitial edema and myocardial fibers were arranged irregularly,and inflammatory cells were infiltrated.Myocardial tissue damage recovered at the 8th week.2.Gene expressionThere was no significant difference in the expression of Akt and GSK3β mRNA in myocardial tissue samples taken at three times in the Sham group(P>0.05);Akt m RNA expression: Compared with the Sham group,the Akt mRNA expression level in the MI group was significantly lower when the samples were taken at 4 and 8 weeks(P<0.05);Compared with MI group,the expression level of Aktm RNA in MI+IRB group increased significantly at 4 and 8 weeks(P < 0.05);GSK3βmRNA expression:Compared with Sham group,MI group was 4 The expression increased at weeks and 8weeks(P<0.05).Compared with the MI group,the expression of GSK3β in the MI+IRB group increased at 4 weeks and 8 weeks,and the difference was statistically significant(P<0.05).3.Protein expressionThere were no significant differences in p-Akt/Akt and p-GSK3β/GSK3β in myocardial specimens taken at different times in the Sham group(P>0.05);compared with the Sham group,the phosphoric acid levels of Akt and GSK3β in the MI group at4 and 8 weeks The expression level of Hg was significantly reduced(P<0.05);compared with the MI group,p-Akt/Akt and p-GSK3β/GSK3β were significantly increased in the MI+IRB group at 4 and 8 weeks(P<0.05).ConclusionThe application of irbesartan helps to reduce the hypoxic injury of rat myocardial tissue,inhibit cell apoptosis,and protect myocardial tissue,and its possible mechanism is to activate the PI3K/Akt/GSK3β pathway to enhance Akt,GSK3β phosphorylated expression level.Moreover,the expression levels of Akt and GSK3β have a certain time effect.