1,2,3,4,6-O-Pentagalloylglucose (PGG) Inhibits The Growth Of NPC 5-8F Cells And Its Key Molecules

Objective: Nasopharyngeal carcinoma(NPC)is characterized by its obvious regional characteristics,high incidence rate,and poor therapeutic effect in the middle and late stages.1,2,3,4,6-O-pentagalloylglucose(PGG)is a kind of naturally hydrolyzable polyphenol compound in fruits and Chinese herbal medicines.It has been found that PGG has multi-target anticancer effect.The aim of this study was to investigate the biological effects of PGG on NPC 5-8F cells and the key molecules involved.Methods:(1)MTT method was used to analyze the viability of NPC 5-8F cells.(2)Flow cytometry was used to detect the effect of PGG on cell cycle distribution and apoptosis of NPC 5-8F cells.(3)Immunofluorescence staining was used to analyze the expression and localization of HURP in NPC 5-8F cells.(4)Western blot was used to detect the expression of cell proliferation-and apoptosis-related genes in PGG treated NPC5-8F cells.Results:(1)PGG inhibited the activity of NPC 5-8F cells in a time and dose-dependent manner.(2)Low dose PGG blocked 5-8F cells in S phase and high dose PGG blocked 5-8F cells in G1 phase.(3)PGG induced apoptosis of NPC 5-8F cells in a time and dose-dependent manner.(4)HURP was located on the spindle of mitotic cells.(5)PGG dose-dependent down regulated the expression of HURP,NF-κB p65,Survivin and Bcl-2 in NPC 5-8F cells.25 μM PGG upregulated Cyclin D1 expression.PGG of 50 μM and 75 μM down regulated the expression of Cyclin D1.In addition,PGG dose-dependent up regulated the expression of Bax in 5-8F cells,and the ratio of Bcl-2/Bax decreased.Conclusion: PGG inhibited the growth of NPC 5-8F cells,which was related to the up-regulation of Bax,down-regulation of HURP,NF-κB p65,Survivin and Bcl-2.