Study On Cell Proliferation Around Two Kinds Of Echinococcus In Mouse Liver At Different Time Points

Objective: To explore the proliferation of cells around Eg(echinococcus granulosus)and Em(echinococcus multilocularis)in mice liver at different time points.Methods: The protoscolices of echinococcosis were extracted from the liver tissues infected with Eg and Em,and then cleaned,sterilized,purified and identified.To establish a comparative experimental model of hepatic cystic echinococcosis and alveolar echinococcosis in mice.Ed U solution was prepared and injected into mice on time.The tissue around echinococcosis was obtained and sectioned.HE staining: paraffin sections were stained with hematoxylin-eosin and observed under inverted microscope.Ed U staining: After frozen sections were treated,Click Additive Solution was prepared and Ed U was labeled.The proliferation of cells around Echinococcus was observed under fluorescence microscope.The clinical specimens of patients with hepatic echinococcosis were compared by HE staining.proliferating cells count and statistical analysis.the number of proliferating cells was counted and statistically analyzed by Image J(1.8.0)software and SPSS(25.0)software.Results: HE staining showed that there were two layers of cell infiltration zone around echinococcus in the two groups,one on the side close to echinococcus and the other on the side close to liver tissue.The two layers of cell infiltration zone inside and outside Em were irregular and discontinuous,while the cell infiltration zone around Eg was regular and continuous.Fluorescence staining showed that the number of proliferating cells of Eg was more and more regular than that around Em at different time points.The number of proliferating cells around Em group and Eg group was 13.73 ± 7.47 and 37.73 ± 7.95 on the30 th day,38.27 ± 19.81 and 69.00 ± 30.29 on the 60 th day,and 70.07 ± 39.83 and 153.27 ±54.28 on the 90 th day,respectively.The number of proliferating cells around 30 days,60 days and 90 days in Eg group was 2.75,1.80 and 2.19 times of that in Em group,respectively.There was significant difference in the number of proliferating cells around 30 days,60 days and 90 days in Eg group and Em group(P < 0.001,P < 0.001,P< 0.001).Conclusion: At different time points of 30 d,60d and 90 d,the distribution of infiltrating cells and proliferating cells around Eg and Em was different,and the number of proliferating cells around Eg was more than that around Em at different time points.It is suggested that the small number of proliferating cells around Em is not conducive to its surroundings around inside and outside the two layers of cells forming a complete cyst wall.