Effects Of High Fat Diet And Exercise Intervention On The Activation Of White Fat Autophagy Related Proteins In Rats

Objective: To explore how high-fat diet and exercise affect the autophagy related proteins in adipose tissue,and to explore the possible mechanism of exercise through regulating autophagy in adipose tissue,so as to provide a theoretical basis for the molecular mechanism of exercise lipid reduction.Methods: Thirty two 3-week-old male SD rats were randomly divided into control group(CS),exercise group(CE),high fat group(HS)and high fat exercise group(HE).CS group and CE group were fed with normal fat diet,HS group and HE group were fed with high fat diet.CE group and HE group took part in the 8-weeks-moderateintensity-aerobic exercise,CS group and HS group didn’d have any exercise project.After the intervention,subcutaneous and testicular white fat of rats were taken.Western blot was used to detect the expression of related proteins in the tissues,and transmission electron microscopy was used to observe the autophagy in the cells.Result: The weight of rats in each group continued to increase,with the percentage of trunk fat and whole body fat in the HS group significantly higher than that of CS(p<0.05),and the percentage of trunk fat and whole body fat in the HE group were significantly lower than that of the HS group(p <0.05).Except for the testicular white adipose tissue in the HS group,autophagosomes were observed by TEM in the other groups.Compared with the CS group,(1)In subcutaneous white fat,the expression of m TOR,ATG13,and P62 was significantly reduced in the HS group(p <0.01);the expressions of ULK1,p-ULK1,Beclin1,p-Beclin1,and LC3-I was significantly increase(p <0.01);(2)In subcutaneous white fat,the expressions of m TOR,ATG13(p<0.05),p-Beclin1,LC3-I,and P62 in CE group was significantly reduced(p <0.01);the expressions of ULK1 and Beclin1 was increased significantly(p <0.01).(3)In testicular white fat,the expression of p-Beclin in HS group was significantly reduced(p <0.01);the expression of ATG13,p-ULK1,Beclin1,LC3-I,and P62 was significantly increased(p <0.01);(4)In testicular white fat,the expression of ULK1,p-ULK1,and p-Beclin1 in CE group was significantly reduced(p <0.01);the expression of ATG13(p <0.05),Beclin1 and P62 was significantly increased(p <0.01).Compared with the HS group,(1)In subcutaneous white fat,the expression of ATG13(p <0.05),ULK1,p-ULK1,LC3-I and P62 in HE group was significantly reduced(p <0.01);the expression of Beclin1 and p-Beclin1 was significantly increased(p <0.01).(2)In testicular white fat,the expression of m TOR,ATG13,ULK1,p-Beclin1 and P62 in HE group was significantly reduced(p <0.01).Compared with the CE group,(1)In subcutaneous white fat,the expression of ULK1 and P62 in HE group was significantly reduced(p <0.01);the expressions of ATG13,Beclin1,p-Beclin1 and LC3-I was significantly increased(p <0.01).(2)In testicular white fat,the expression of m TOR,ATG13,ULK1,Beclin1,LC3-I and P62 in HE group was significantly reduced(p<0.01);the expression of p-ULK1 was significantly increased(p <0.01).Conclusion: 1.High fat diet can promote the initiation of autophagy.This effect is not regulated by inhibiting m TOR phosphorylation of the ULK1-related pathway.Regulatory mechanisms have tissue difference.2.Exercise intervention can promote the start-up of autophagy in white adipose tissue of normal diet rats.This effect is not regulated by inhibiting m TOR phosphorylation of the ULK1-related pathway.Regulatory mechanisms have tissue difference.3.Exercise intervention has a significant effect on the initiation of autophagy in white adipose tissue of high-fat diet rats,which has tissue difference;It promotes the initiation of autophagy in subcutaneous white adipose tissue by inhibiting m TOR phosphorylation of the ULK1 related pathway;inhibits the autophagy initiation of white fatty tissue by inhibiting ULK1 phosphorylation of the Beclin1.