| Objective: In recent years,studies have found that exosomes play an important role in the field of sports science.As a kind of exercise factor,exercise-induced exosomes can regulate multiple tissues and organs such as skeletal muscle,and participate in the regulation of the body’s environmental homeostasis and physiological functions.Exercise-induced fatigue is a complex phenomenon that is affected by factors such as central nerve recruitment neurons and skeletal muscle mechanical movement.It is an important factor affecting athletes’ competitive level.Finding potential diagnostic molecules is the current research value.To this end,this study used high-throughput detection technology to obtain plasma exosomal miRNA expression profiles of college athletes before and after the full 1500-meter freestyle exercise,and screened differentially expressed exosomal miRNAs,and performed functional enrichment analysis on differentially expressed miRNAs.Test fatigue-related physiological and biochemical indicators,and explore the changes in plasma exosomal miRNAs and related fatigue indicators in a full 1500-meter freestyle swimming,to Provide a theoretical basis for finding new molecules related to fatigue..Methods: Thirteen male first-level swimmers from Guangzhou Institute of Physical Education were selected as the experimental subjects to perform a1500-meter freestyle swimming with all their strength,and the subjective motor perception scale(RPE)was used to detect the physical sensation.The elbow venous blood of the tested athletes was obtained before and after the exercise,the exosomes in the plasma were extracted,and the illumina 2x150 bp high-throughput chip detection technology was used to analyze the differential expression of exosomal miRNA in the subject’s body under fatigue state,and screen out the differentially expressed miRNA.Shengxin analysis GO and KEGG database tools screen the target genes and signal pathways regulated by differentially expressed miRNAs.And test fatigue-related indicators: 100-meter segment performance,anaerobic capacity,heart rate(HR),heart rate variability(HRV),serotonin(5-HT),blood lactic acid(Bla),blood urea(BU),serum creatine Kinase(CK),white blood cell(WBC),red blood cell(RBC)and hematocrit(HCT%),as well as measurement of reaction time(RT)and correct rate(ACC)during the execution of the Stroop color word task.All data are statistically analyzed by T test.Results: 1.Successfully extract exosomes from plasma,and use the miRNA high-throughput detection platform to analyze the miRNAs of the two groups of athletes’ plasma exosomes before and after the experiment.It is found that 70 miRNAs have different changes between the two groups,and 45 miRNAs are expressed.Up-regulated,25 miRNAs were down-regulated;after further screening,it was found that there were 5 target genes with P<0.05 and|log 2FC|>1,and the 4up-regulated miRNAs were let-7b-5p,let-7i-5p,miR-126-3p,miR-127-3p,and the other 1 down-regulated miR-10a-5p.2.Perform functional enrichment analysis on five target genes,combined with the analysis of the three database tools of PITA,Targetscan,and miRand,it is found that the target gene miRNA and LTP,VEGF,glutathione and other metabolic pathways,Dopaminergic synapses are related to biological processes such as signal transmission and biological regulation.3.From the fifth 100-meter segment,the performance begins to drop to a stable state.The RPE scales are all above 19(extremely laborious),and the heart rate is close to the theoretical maximum heart rate(HRmax);the maximum output power(PP)and the relative maximum The power decreased,the rate of power decline and the percentage of fatigue increased significantly(P <0.01);the overall HRV value decreased significantly(P <0.01),and the fatigue index,the stress index of the cardiac system,and the heart rate deceleration force were also significant Decrease(P <0.01);ACC decreased,AT significantly increased(P <0.01);central neurotransmitter 5-HT significantly increased(P <0.05),blood lactic acid(Bla),blood urea(BU),serum muscle The values of acid kinase(CK)and white blood cell(WBC)both increased significantly(P <0.01),and the changes in RBC and HCT were not obvious,indicating that the changes in the subject’s body after a full 1500-meter freestyle exercise may be due to fatigue resulting in.Conclusion: 1.After a full 1500-meter freestyle swimming,there are 70 differentially expressed miRNA changes in plasma exosomes,of which 45 are up-regulated and 25 are down-regulated.Five of them(let-7b-5p,let-7i-5p,let-7i-5p,miR-10a-5p,miR-126-3p and miR-127-3p)miRNAs with significant changes may be involved in the regulation of LTP,VEGF,glutathione metabolism pathways,dopaminergic synapses and signal transmission,biological regulation and other organisms Learning process.2.Relevant fatigue indicators occur significantly after the full 1500-meter freestyle exercise,the body may have fatigue,and there is research value with the characteristics of exosomal miRNA changes. |
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