Preparation Of Nano-Ag-Zn Lattice Coating And Its Antibacterial And Cell Proliferation Properties

Nano-silver and nano-zinc materials are widely used in the medical and health industry due to their good antibacterial property.Clinical experiments have proved that the Ag-Zn mixed coating could inhibit the growth and reproduction of various pathogenic bacteria,such as Escherichia coli and Staphylococcus aureus.Investigation has shown that the Ag-Zn ions can interact to generate a biologically coupled electromagnetic field,which could promote the division,proliferation and re-growth of damaged tissue cells in the human body.It is beneficial to the healing of wound tissues and can be used as a new type of material in medical and health equipment.In this paper,the nano-silver and nano-zinc materials are designed in a dot matrix form.The ultra-thin porous anodic aluminum oxide(AAO)double pass template is used as the auxiliary substrate to manufacture the Ag-Zn dot matrix coating.Three different dot matrix forms(double-layer dot matrix,layer-dot matix,and hybrid layer)are manufactured by The DC-AC bidirectional RF magnetron sputtering.After the sputtering,the AAO template is stripped and removed from the coating.The coating properties(the surface morphology,element type and content,roughness)are carried out by scanning electron microscope,atomic force microscope,energy dispersive X-fluorescence spectrometer,X-ray energy spectrum analyzer and contact angle measuring instrument.The typical coating shows that the diameter of the dot is 200 nm,the thickness is 50-200 nm,and the separation distance of each dot is 400 nm.The microstructure of Ag-Zn coating is affected by the sputtering parameters,such as injection time,filling gas flow rate,and gas pressure.However,with the increase of sputtering time,the dot sequentially appears 5 forms of accumulation:,the hill-shaped,the spherical,the mountain-shaped,the pasty,and the scum-shaped.It can be found that if the thickness of dot is 100nm(the unit cell shape is spherical),the obtained surface is regular,smooth and highly ordered.The best morphology of the coating shows spherical dot,with a roughness of 52 nm.Meanwhile,the contact angle of the coating is 103.6°,which is a hydrophobic surface with a certain self-cleaning function.To explore the antibacterial and cell proliferation properties of the coating,Escherichia coli(Gram-negative bacteria)and Staphylococcus aureus(Gram-positive bacteria)were selected.The bacterial colony filming method was used to test the antibacterial performance of different dot matrix coatings.It was found that the nano-Ag-Zn dot matrix coating can combine the antibacterial properties of nano-silver and nano-zinc materials,and can better produce both types of bacteria.Extremely strong bacteriostasis(both bacteriostatic efficiency is more than 99.5%);through cell comparison experiments,the x CELLigence cell function analysis method was selected to detect the impact of nano Ag-Zn dot matrix coating on the biological activity of human immortalized epidermal cells(Hacat),The experimental results show that: an appropriate amount(coating thickness <50nm)of nano-silver materials can promote cell proliferation and growth,and excessive(coating thickness>100nm)nano-silver materials can promote cell proliferation and growth for a short time and then inhibit;(Coating thickness <5nm)nano-zinc material promotes cell proliferation and growth,an appropriate amount(5nm<coating thickness<10nm)of nano-zinc material inhibits cell proliferation and growth,excessive(coating thickness>10nm)Nano-zinc materials will directly inactivate and kill cells.Finally,the structure with silver layer thickness 95 nm and the zinc layer thickness 5nm.shows best performance on the promotion of the Hacat cells growth..Therefore,by designing and preparing nano Ag-Zn dot matrix coatings with certain parameters,it has good performance in inhibiting bacterial reproduction and promoting cell proliferation and growth,and can be used as a new functional material in the medical and health industry.