Design And Antibacterial Mechanism Of ZnO/Cu₂O Composite Antibacterial Materials

As pathogenic microorganisms such as bacteria and molds seriously affect people's health and quality of life,and even pose a huge threat to people's life safety,the research of safe,efficient and environmental friendly antibacterial materials have important practical meaning.Cu-based antibacterial materials not only have high efficiency antibacterial properties,but also have lower cost compared with Ag-based antibacterial materials;Zn-based antibacterial materials have the advantages of good stability,resistance to drug resistance,and good biocompatibility,so both have good and broad application prospects in the field of antibacterial.In this paper,ZnO with good stability and excellent biocompatibility was loaded on Cu₂O with high antibacterial performance but easy to burst release of antibacterial ions to obtain a composite antibacterial material,which can not only relieve the easy burst release behavior of Cu₂O antibacterial ions,but also improve the antibacterial properties of ZnO.In this experiment,the liquid-phase reduction method was used to prepare Cu₂O,and the liquid-phase precipitation method was used to load ZnO on the surface of Cu₂O to obtain ZnO/Cu₂O composite antibacterial material.By SEM,EDS,BET,XRD,FTIR,TEM,XPS and the basic form of ZnO/Cu₂O composite antibacterial material,chemical composition and crystal structure analysis.UV-vis spectroscopy and PL spectroscopy were used to analyze the optical properties of ZnO/Cu₂O composite antibacterial materials,which showed that Cu₂O can effectively reduce the band gap energy of ZnO in ZnO/Cu₂O composite antibacterial materials,improved the efficiency of electronic transitions,and suppressed electrons and the reorganization of cavities promoted the production of ROS and improved antibacterial properties.E.coli and S.aureus were used to test the antibacterial properties of the composite antibacterial material,and it was found that the ZnO/Cu₂O composite antibacterial material had good antibacterial properties against both bacteria.At the same time,this article showed that the sustained release dissolution experiment showed that loading ZnO on Cu₂O can effectively reduce the release rate of Cu²⁺to achieve the purpose of long-lasting antibacterial.Through ion dissolution,ROS antibacterial experiment and mechanical damage experiment,the antibacterial mechanism of ZnO/Cu₂O composite antibacterial material was discussed from the material level.The results showed that ZnO/Cu₂O composite antibacterial material not only has the above three antibacterial mechanisms,but also after loading ZnO,Cu²⁺was slow release,increased in ROS and rough appearance of the material,so the ZnO/Cu₂O composite antibacterial material has better antibacterial properties.Finally,in the bacterial level,this article used SEM to characterize the morphology of the bacteria treated with the ZnO/Cu₂O composite antibacterial material,and preliminarily analyzed the morphological changes of the bacteria;furthermore,the detection of lipid peroxidation,protein leakage,respiratory chain dehydrogenase activity and bacteria in vitro fluid ion was further analyze the internal damage of the bacteria,further analysis of the reasons why the composite antibacterial material inactivates the bacteria from the inside of the cell.