Study On Anti-Aging Effect Of Blackcurrant Extract On Drosophila Melanogaster

Aging is a natural process that can not be resisted,and anti-aging has been a popular research topic.It has been found that blackcurrant extract has antioxidant,hypolipidemic,and antitumor physiological activities in many studies.In this paper,we investigated the antiaging effects and the mechanism of blackcurrant extract in Drosophila melanogaster through network pharmacology prediction and molecular docking simulation.In this study,drosophila was used as a model to review the results of different concentrations of blackcurrant extract(0.2 mg/m L,1 mg/m L,2 mg/m L)on delaying senescence and prolonging the lifespan of Drosophila melanogaster.The results showed that blackcurrant extract extended the lifespan and improved locomotion but did not affect the weight and food intake.In this paper,drosophila under different stress conditions was established to investigate the effect of blackcurrant extract on stress tolerance.The results showed that blackcurrant extract improved the survival time of drosophila under various stress conditions(oxidative stress,heat stress,starvation stress).At the same time,blackcurrant extract improved the intestinal microbial environment,enhanced the intestinal barrier function,inhibited the abnormal proliferation of intestinal stem cells,and increased the number of autophagic lysosomes in the intestinal tract of drosophila.As for RNAi drosophila,it was found that the lifespan of all RNAi fruit flies was shorter than that of the wild type w1118.Moreover,the exercise ability of autophagy related RNAi drosophila was measured,and the results showed that the locomotion ability of Atg5 RNAi and Atg8 a RNAi drosophila was not improved by the blackcurrant extract intervention.In this study,we further explored the molecular mechanism of blackcurrant extracts’ anti-aging.The results showed that blackcurrant extract could significantly increase the m RNA expression of CAT and SOD,reduce the gene expression of PI3 K,Akt-1 and m TOR,and significantly increase the downstream autophagy genes Atg1,Atg5,Atg8 a and Atg8 b,but it did not affect the gene expression of sir-2.These results indicated that the anti-aging effect of blackcurrant extract was the inhibition of PI3K-Akt pathway and m TOR pathway,and activation of autophagy pathway,but not dependent on dietary restriction.Meanwhile,the improvement of the m RNA expression of Hsp70 indicated that the stress resistance of blackcurrant extract in an adverse environment may be due to the action of Hsp70.These results provided a theoretical basis for blackcurrant to develop anti-aging functional food.