Establishment Of Propagation And Regeneration System Of Moso Bamboo And PhPPO And PhE2F Family Expression In Moso Bamboo

Moso Bamboo(Cunninghamia lanceolata(Lamb.)Hook),important economic bamboo species in China,both extremely important ecological and economic value.Ecological values reflected in Moso bamboo in southern China,as an important species,its growth and cover a wide area,and generate economies of scale,and to some extent have a positive effect on water conservation in regulating climate;their economic value lies in,bamboo as a forestry resource in itself.So have some social value.Due to the large demand for bamboo and cut spamming is serious,vegetative propagation significantly to this tradition can not meet the needs of its vast,vegetative propagation speed,in addition to bamboo itself is quality defects,such as perishable.You can go through the gene technology to improve it,but the transformation of bamboo although there are a number of success stories,but are limited to the mushrooming of bamboo,for the bamboo,such as bamboo,although much has been done sequencing successful but had not been translated into the success of precedent.This is also significant constraints on the bamboo research and development work.Bamboo propagation experiment to solve system,regeneration,as well as related gene transcription mechanisms,to improve bamboo quality and quantity as final objective,innovative use of chlorine disinfection and improvement of medium composition,and red light,I get a variety of results,hoping for the tissue culture techniques of Phyllostachys pubescens and transgenic technology to establish a foundation.Some discovery in building of propagation system:1.After soaking 1 day,7 hours at 0.01mol/L concentrations of chlorine,uncontaminated and germination rate of the seeds of Moso bamboo respectively up to 96.23±0.19%and 74.00±1.89%.2.The cytokinin 6BA and TDZ in relation to other hormones,proved to be more suitable for bamboo propagation of cytokinin.TDZ best bud differentiation and its initial propagation index reached 2.98±0.02;6BA is more conducive to the growth of buds,which induce bud grows fast,and induce bud in the same period compared to other hormones is one of the longest.3.Sample 2mg/LTDZ hormone found in relation to MS medium,Division of MS1 medium of more effective.4.6BA after combination with TDZ,with MS1 medium as medium,found in MS1+3mg/L6BA+lmg/L conditions,propagation index reached 7.52+1.77.5.In the rooting experiment,discovery will shoot into buds per plant,and after stripping the buds to lure,finally found in MS 1+2mg/LIBA+0.3mg/LTDZ conditions,its root works best,and differentiation of buds at the bottom again.Some discovery in building of regeneration system:l.Found that the most suitable conditions for inducing callus is:MS1+4mg/L2,4D+0.1mg/LZT.after 15 days in the dark culture,callus induction rate of 34.88±2.81%.2.In the subculture,hormone treatments,and found 2mg/l 2,4D is the best subculture medium hormones.3.In the rooting experiment,discovered MS+3mg/LNAA hormone treatment is the best rooting condition of callus,and the rooting rate and survival rate 47.71±3.98%and 34.81±2.39%.4.In order to promote the differentiation of rates,this red light treatment and training and composition improvements found in MS1+3mg/LNAA condition moves after 15 days in the Red to dark conditions,the root index reached 8.56+1.22,has the best a divisive effect,callus was yellowish color glossy,vitality.5.During the process of bud induction,callus into small,found the little callus relative to large callus,more conducive to survival.Steroid-induced,under 5mg/L ZT found calli in the 10 days after germination and sprouting.6.Survival under roots and shoots of embryogenesis,in natural light training with the next,begin to turn green in a week,but all begin Browning in two weeks.Some discovery in gene expression:1.Bamboo E2F gene family evolution,based on the amino acid sequence and structure analysis 3 subtypes were identified 12 E2F protein family.2.Through the sequence compared to the conservative structure territory analysis,discovered bamboo E2F the family Asian family E2F has highly conservative DNA the binding region,simultaneously E2F and DEL have conservative Leucine Zipper,Marked the box two conservative regions.In addition,the system growth number showed south drawing up the mustard,in the paddy rice and the bamboo three species,discovered the bamboo in the mustard has 6 pair of direct line homologous genes south drawing up,2 pair of collateral branch homology gene.Has 8 pair of,the direct line homologous gene in the bamboo,a pair of collateral branch homology gene;This also indicated three species have the widespread common ancestor in the E2F genome team.3.PhDPB in the bamboo shoots to form subfamily(PhDPB1,PhDPB2 and PhDPB3)is a key transcription factor stem development.PhE2F2 is the apical meristem formed an important transcription factor.4.PhDPA1 with high expression in the differentiation of callus,not expressed in undifferentiated callus,indicating E2F2 gene in differentiation of the callus of Phyllostachys pubescens in,adjust the Browning mechanism.5.PhPPO1 is likely to be involved in the organization of the wounded bamboo Brown of the process of globalization.Phppo2,PhPPO3 and will not cause more injuries bamboo organization of welfare.