The Study Of The Camellia Japonica Winter Buds Germination And Tissue Culture

(Camellia japonica var.Decumbe)belongs to a variant of Camellia,（Camellia japonica var.Decumbe）cold resistance,is the introduction of our important flower ornamental tree species.In order to quickly breed Camellia japonica var.Decumbe,strengthen the Camellia japonica var.Decumbe seedling management,improve its cold resistance,In this paper,based on the research on the tissue culture technology system of（Camellia japonica var.Decumbe）,This article was carried out（Camellia japonica var.Decumbe）winter bud germination characteristics,and Effects of Low Temperature Stress on Physiological and Biochemical Characteristics of Winter Bud and Leaves。Get the following result:（1）In three different time periods,20℃ is the most suitable for the expansion of Camellia japonica top buds 20℃,the Camellia japonica top buds first sprouted in20℃,the highest sprouting rate in 20℃,At the same time 20℃ is the most suitable for the expansion of Camellia japonica lateral buds,the Camellia japonica lateral buds first sprouted in 20℃,the highest sprouting rate in 20℃.（2）In the case of three different freezing treatments,the sprouting rate was the highest at 20 ℃ for Frozen at 0℃ for 15 days,The survival rate of Camellia japonica was 100% after Frozen at 0℃ for 15 days,The survival rate of Camellia japonica)was 66.7% after Frozen at-5 C for 15 days and he survival rate of Camellia japonica was 33.3% after Frozen at 0^-10° C for 15 days.（3）Trends of Physiological and Biochemical Indexes of Different Low Temperature Stress:The changes of SOD activity in Camellia japonica leaves and buds at 0 ℃,-5 ℃ and^-10 ℃ low temperature stress showed a tendency to decrease first and then decrease.After 0 ℃,-5 ℃ and^-10 ℃ low temperature stress,The change of soluble protein content was the trend of decreasing first and then rising and then decreasing slowly,Change is relatively small;After changing at 0 ℃,-5 ℃ and^-10 ℃,the change trend of POD was the trend of rapid decline and then rising slowly.The trend of change was relatively large.（4）Camellia japonica var.Decumbe buds、leaves the best disinfection program is:buds、leaves with water for 2-5h,with 75% alcohol bubble for 30 s,and then sterile distilled water rinse 1-2 times,and then 0.1% 1Hgcl2 disinfection 8min,and then with the Bacteria distilled water rinse 5-7 times.The survival rate was 99.5%.（5）The most suitable medium for induction of Camellia japonica var.Decumbe proliferation of : MS + 1.5 mg· L^-1 + 0.1 mg ·L^-1,the inductio rate was 85%,the average bud length was 1.6cm,the highest The buds optimum hormone combination was MS+NA A0.2mg·L^-1 + IBA0.5mg·L^-1 + 3mg·L^-1,the multiplication factor was 2.63 and the effective germination rate was 86.7 %.（6）The best hormone combination induced by the leaves of callus is:MS+6-BA2mg·L^-1+ IBA0.5mg·L^-1 and the induction rate was 93%.Leaf callus proliferation of Best hormone ratio was:MS+6-BA2mg·L^-1 + NAA0.2mg·L^-1,the optimal hormone combination of callus-induced adventitious buds was: MS+0.5 mg·L^-1IBA+5mg·L^-16 BA,MS+ 0.2mg·L^-1NAA+3m g· L^-16-BA.（7）Rooting the best hormone program:1/2 MS + NAA1.0mg· L^-1.