Study On Production Technology,quality Standard And Clinical Efficacy Of Jiawei Pingwei Oral Liquid

Jiawei Pingwei Oral Liquid is a compound mixture used to treat damp-heat diseases in poultry.It is made on the basis of the traditional Chinese veterinary theory knowledge combined with modern Chinese pharmacological research,on the basis of the classic prescription Pingwei Powder.appetizing.This prescription is an effective prescription screened out through clinical comparison,and has no adverse reactions so far.The main research of this subject is: the production technology of Jiawei Pingwei Oral Liquid,the quality standard and the preliminary exploration of clinical efficacy.The production process research mainly includes extraction process,purification process,molding process and small test verification.In the extraction process,single factor test and orthogonal test were used to screen out the best extraction process for volatile oil.The medicinal materials were processed into small pieces of 1～2 cm,8 times of water,decoction for 10 hours,and the medicinal residues were mixed with the remaining medicinal materials.,decoction with water,the optimal extraction condition is 8 times of water,and the extraction is performed 3 times,1.5 hours each time;in the purification process,the impurity removal effect of high-speed centrifugation and alcohol precipitation method on oral liquid is compared first,and the orthogonal The experiment determined that the best alcohol precipitation process is to concentrate to the relative density of the liquid medicine 1.15(60 ℃,60% ethanol concentration,let stand for 24 hours,and then investigate the water precipitation process.There is no more precipitation;the dosage of co-solvent Tween-80,the p H range of the medicinal solution,the dosage of preservatives and the sterilization process were investigated successively in the molding process.The optimum concentration of Tween-80 was determined to be 1.5%,and the p H value was between 3.0 and 6.0.Sodium benzoate was selected as the preservative,with a concentration of 0.3%,and sterilized at100 °C for 30 minutes;the process verification was carried out on ten batches of small tests.,which proves that the production process is stable and feasible,and the crude drug ratio of the oral liquid is determined to be 1 g/m L.The study of quality standard includes the TLC identification of main medicinal materials and the content determination of main components.Thin layer chromatography(TLC)was used to identify Atractylodes macrocephala,Aucklandia,Sophora flavescens,tangerine peel and Fructus aurantii in Jiawei Pingwei oral liquid.The spots in the chromatogram of the test sample were clear and round,and the negative control had no interference.It can be used as a TLC identification method;The contents of naringin and matrine in oral liquid were determined by high performance liquid chromatography(HPLC).The methodological results were in line with the provisions of the Pharmacopoeia.Ten batches of samples were tested by this research method.The content standard of naringin and matrine was specified according to the test results.It is tentatively determined that the content of naringin and matrine per ml of this product should not be less than 2.22 mg and 0.57 mg respectively.The preliminary exploration of clinical efficacy is to select 36,000 12-day-old 817 broilers with natural disease.The main manifestations are: decreased feed intake,long strips of manure or over-feed manure(feces contain undigested feed),and adenomyosis can be seen in autopsy.Stomach edema and ulcers of varying degrees.The experimental chickens were randomly divided into four groups,namely the oral liquid high-medium-low-dose group and the control group.The high-medium-low-dose group was given 1500 m L,750 m L and 250 m L of Jiawei Pingwei oral liquid every day,and the control group was not given any drug.Combined with amoxicillin soluble powder 500 g for 5 days.The treatment effect showed: the feed intake of the oral liquid high-dose group increased to about the standard level,the long or overfeeding dung decreased,the symptoms of autopsy were relieved,the feed intake of the low-dose group and the control group gradually decreased,and the adenomyosis was seriously edema..It can be seen that Jiawei Pingwei Oral Liquid has a therapeutic effect on damp-heat in the spleen and stomach of poultry.This study clarified the production process of Jiawei Pingwei oral liquid,formulated the quality standard,verified the clinical therapeutic effect,and provided data reference for further pilot study,pharmacodynamic study and application of new veterinary drugs.