| Arundo donax cv.Lüzhou No.1 has high forage,production and ecological value,and has a broad application prospect.The research on tissue culture and regeneration of Lüzhou No.1 is of great significance to its resource development and sustainable utilization.Taking the stem segments,buds,leaves and stem nodes of Lüzhou No.1 as test materials,a complete and efficient tissue culture propagation system of Lüzhou No.1 was established through the rational selection of explants and the optimization of disinfection conditions,screening induction,proliferation and rooting medium,seedling refining and transplanting.The results are as follows:(1)April is the best time for tissue culture and explants of Lüzhou No.1,and the stem node(0.4 ～ 0.6 cm)at the front of the tender branch at the vigorous growth part of Lüzhou No.1 is the best explant.The suitable disinfection method for stem nodes is: 75%ethanol for 30 s + 2% Na Cl O for 6min,the highest germination rate is 87.8%,and the pollution rate and browning rate are low,which are 9.4% and 2.8% respectively.(2)MS medium had significant induction effect on stem explants of Lüzhou No.1.The optimum primary medium was MS + 6-BA 5.0mg/L + 2,4-D 0.2mg/L + IBA 1.5mg/L+ NAA 0.2mg/L,the induction rate was 92.22%,and the proliferation coefficient was 3.32;The height of bud is 7.7 cm.The optimum proliferation medium of Lüzhou No.1 was MS+ 6-BA 6mg/L+ 2,4-D 0.2mg/L + IAA 1.0mg/L + NAA 0.1mg/L,and the proliferation coefficient was 7.29.The rooting culture of adventitious buds was MS + IBA 5.0 mg/L +NAA 0.9 mg/L.River sand: peat soil: Perlite=(2:1:1)(V:V).After 20 days of transplanting,the survival rate is 96.67% and the average plant height was 24.27 cm.(3)In the proliferation process of Lüzhou No.1 shows that the types of plant growth regulators will have an impact on the proliferation coefficient of Lüzhou No.1,and the primary and secondary order is 6-BA,2,4-D,IAA and NAA.Among them,the effect of factor 6-BA is very significant(P<0.01). |
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