Domestication And Transplanting Of Tissue Culture Seedlings Of Ruby Seedless

In grape plantlets transplanting process, the environment changed dramatically, little attention, or master transplanting techniques of tissue culture will lead to massive death. Therefore, the master grape plantlets transplanted relevant theories and techniques and the survival rate was found and demonstration of what measures can increase the survival rate of transplanted tissue culture seedlings is very important.This study, based on previous seedless grape breeding technology, tissue culture seedlings of ruby seedless were used as the experimental materials. The normal intact seedlings were cut to 2cm long stems with leaves and a bud of a segment, subculture, and strong rooting, transplanting domesticated. By using different growth regulators, observe different height tissue culture, cultivation in different growing media and different exogenous nutrient solution for transplanting plantlets survived the impact of domestication, derived data for analysis. And further improve the culture seedlings, transplanting until all aspects of the field hardening and further improve the hybrid offspring of seedless grape seedling rate, in terms of transplanting tissue culture techniques and other domesticated conducted research to be obtained suitable practical techniques to further improve the efficiency of breeding seedless grapes.Taking these four kinds of seedling rate results seedless grape varieties analysis and comparison, we can see that the rate for the greatest impact seedling plant growth regulator auxin (IAA), followed by 6-BA, ABA and GA 3, adding a certain the amount of growth hormone can promote embryo germination to improve seedling rate.Domestication process of transplanting a greater impact on several aspects of (tissue culture rooting and plant growth conditions, transplanting container, transplanting and spraying liquid matrix, etc.) carried out a study to establish a suitable transplanting seedlings acclimated operating procedure:Step One:First select the subculture medium (1/2×MS+ 0.3 mg.L'IAA rooting, place it in between the light intensity increased cultivation closed hardening 7-14 days; Step two:Use the appropriate mode of operation out 10 cm high seedling transplanting to air-M1 matrix (vermiculite:peat:coconut husk=1:4:1) a 18 cm× 16 cm nutrition bowl, then use watering can fitted with sterile water, spray the entire tissue culture. The third step:Use 1/10×MS macronutrients+1×MS medium solution other ingredients as a nutritional liquid watering just transplanting the seedling, drenched. Then sealed with a transparent plastic disposable cups seedling. Mark, hardening chamber hardening domesticated. Step Four:Nutrition seedling moved within 30 days of acclimatization greenhouse, greenhouse nursery moved in, listing. Step Five:March planting next spring field. Operating procedures refinement, with practical help to improve operability while transplanting seedlings acclimated seedling rate.