| Arundo donax is adaptable,high biomass of perennial herbaceous plants,got the good utilization value in many field,such as forage,biomass energy,water&soil conservation plant,etc.The article will mainly analyze the genetic characteristics and agronomic traits of Arundo donax which collected from different regions and countries.On the basis of analysis of the germplasm resources,aimed at cuttage propagation of Arundo donax,the research focus on the effects of axillary bud germination,seedling and root growth in different temperature and different positions,the effects of Arundo donax seedling growth and root activity by the different nitrogen fertilizer.In terms of the comprehensive utilization of Arundo donax,studied in taking Arundo donax to cultivate Pleurotus ostreatus&Auricularia,at the same time,analysis and evaluation of nutrition value of their fruitbody,the main results of the study are as follows:1.Collecting 8 A.donax resources from Africa and different regions of China,amplified and measured the ITS sequence of the rDNA respectively.Through the phylogenetic tree and analysis of genetic distance,the results show that the Lz 2,Lz 3,Lz 4,Lz 5 are close in genetic relationship and the clustering of then as a branch,Lz8 is close to Arundo donax var.versicolor in genetic relationship,the other 2 were clustered into independent branches,distantly related.2.The plant height,leaf length,leaf number,leaf width,stem diameter,tiller quantity,internode spacing,cluster weight and plant weight were measured of different Arundo donax in the same place.By analyzing and comparing the agronomic traits of various Arundo donax cultivars,the difference of Arundo donax from different areas was explored.Arundo donax varieties from different areas,such as the number of internodes,tillering,weight of single plant,weight of single clump,photosynthetic parameters,carbon and nitrogen ratio have significant difference,rich in variation,variation coefficient from 14.55%一 79.74%,among them,maximum variation coefficient is the weight of single plant,minimum variation coefficient is leaf amount.3.The axillary bud germination rate of A.donax were studied by using the different temperature artificial climate room.The result show us that,temperature has significant impact on the axillary bud germination and root growth of Arundo donax.In addition,different varieties of Arundo donax,the axillary bud germination rate is also different.The optimum temperature of Lz 1 axillary bud germination is 25～30℃,the germination rate is 96.67～98.67%,germination period is about 10 days;with the condition of 25～30 ℃ the germination rate of Lz3 is 80～82.67%.After 60d under 30 C,root total length is 60.89 cm,root surface area of 1.86 cm2,root tip number is 775 of the seedlings of Arundo donax.As a result,the optimum temperature of Arundo donax cuttage propagation is 25～30℃.4.In the experiment of nitrogen effect on the A.donax,12g,15g,18g and 21g of nitrogen fertilizer were application respectively.The growth of aboveground parts was recorded and the root activity of Arundo donax seedlings was studied after 2 months.The study results show that the different nitrogen have a significant impact of Arundo donax seedling biomass and root activity,with the increase of nitrogen,seedling biomass and root activity is up to a certain degree then down gradually.According to seedling biomass and root activity,Arundo donax cuttage propagation,the seedling growth period the best nitrogen fertilizer usage is 375 kg/hm2.5.Pleurotus cultivation by Arundo donax shows that different culture medium formula has a significant impact on mushroom mycelium growth.The best formula of Arundo donax cultivation Pleurotus as follows:Arundo donax 78%,wheat bran 15%,corn flour 5%,and calcium carbonate 2%.The biological conversion rate of Arundo donax and Miscanthus floridulus cultivation Pleurotus were 89.72%and 88.12%respectively.The protein of Arundo donax cultivation Pleurotus is 32.6%,24.29%for total amino acids,essential amino acids accounted is 35.32%.Through the analysis of CS and AAS,the first limiting amino acid of Arundo donax cultivation Pleurotus is Methionine;amino acid index(EAAI)is 0.82.Through the statistic analysis,no obvious difference was found between Miscanthus floridulus and Arundo donax cultivation pleurotus.6.The research of Arundo donax cultivation polytricha shows that different culture medium formula has a significant impact on mycelium growth rate.The best formula of Arundo donax cultivation Auricularia polytricha is:Arundo donax 58%,Dicranopteris dichotoma 20%,wheat bran 15%,corn flour 5%,and gypsum 2%.The biological conversion rate is 112.83%,protein is 15.6%,11.77%for total amino acids(essential amino acids 38.66%).Through the analysis of CS and AAS,the first limiting amino acid is methionine;amino acid index(EAAI)is 0.87,as a good source of protein.Through the statistic analysis,no obvious difference was found between Miscanthus floridulus and Arundo donax cultivation Auricularia polytricha. |
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