Effects Of UV-A Supplementary Light Treatments On Physiological Metabolism Of Acanthopanax Senticosus

Acanthopanax senticosus(Rupr & Maxim)Harms is a traditional Chinese medicine with anti-inflammatory,anti-tumor and other pharmacological effects.As one of the important reasons for inducing plant photosynthesis and photomorphogenesis,UV-A radiation can activate the plant defense system and complete the metabolic response,thus affecting the physiological metabolism and medicinal activity of A.senticosus.In this study,the effects of enhanced UV-A radiation on the physiological indexes,main medicinal active components and primary metabolites of A.senticosus were systematically studied by supplementing light with different intensities of UV-A radiation.The physiological metabolic response mechanism of A.senticosus to UV-A radiation was revealed,and the following conclusions were obtained :(1)Different intensity of UV-A radiation had a significant inhibitory effect on POD activity of A.senticosus roots,CAT activity of stems and leaves and SOD activity of each part,and promoted CAT activity of A.senticosus roots.With the increase of UV-A radiation intensity,the POD activity of A.senticosus stems increased gradually,and the POD activity of A.senticosus leaves decreased first and then increased gradually.With the increase of UV-A radiation intensity,the CAT activity of A.senticosus stems gradually decreased,and the CAT activity of A.senticosus leaves was significantly inhibited,but there was a trend of gradual recovery.(2)Compared with the control group,there was no significant change in the overall growth under L1 treatment,but it could promote the accumulation of soluble sugar in rhizomes and the accumulation of soluble protein in leaves.L2 treatment significantly inhibited the thickening of stems and petioles and significantly inhibited the accumulation of soluble sugar in roots and stems of A.senticosus.L3 treatment significantly promoted the increase of plant height and the thickening of stems and petioles,and significantly promoted the accumulation of soluble protein and soluble sugar in the roots of A.senticosus,but significantly inhibited the growth of leaf area.L4 treatment significantly promoted the increase of plant height,the accumulation of soluble sugar in the rhizome of A.senticosus and the accumulation of soluble protein in various parts of A.senticosus,but significantly inhibited the thickening of stem and the growth of leaf area.(3)L1 treatment could promote the accumulation of Eleutheroside B,Isofraxidin and Chlorogenic acid in the roots of A.senticosus,and significantly inhibit the accumulation of five components in stems and leaves;L2 treatment could promote the accumulation of Isofraxidin in roots,increase the content of Eleutheroside B and Hyperoside in stems,but significantly inhibit the accumulation of five components in leaves.L3 treatment can promote the accumulation of five components in the roots of A.senticosus,and promote the accumulation of Eleutheroside E and Hyperoside in stems,but significantly inhibit the accumulation of five components in leaves.L4 treatment could promote the accumulation of five components in the roots of A.senticosus,and promote the accumulation of Eleutheroside B and Hyperoside in stems,but could significantly inhibit the accumulation of Eleutheroside E and Chlorogenic acid in stems and leaves.(4)Based on the GC-MS platform,non-targeted metabolomics analysis was performed on the control group and the treated A.senticosus root samples.Finally,54 differential metabolites were obtained in the CK-L1 group,44 differential metabolites were obtained in the CK-L2 group,51 differential metabolites were obtained in the CK-L3 group,and 54 differential metabolites were obtained in the CK-L4 group,a total of 81.Through the enrichment analysis of metabolic pathways,8 important metabolic pathways were screened out,namely : Glyoxylate and dicarboxylate metabolism,Galactose metabolism,Aminoacyl-t RNA biosynthesis,Alanine,aspartate and glutamate metabolism,Butanoate metabolism,Glycine,serine and threonine metabolism,Citrate cycle(TCA cycle)and Sulfur metabolism.L3 treatment can promote the accumulation of D-Glucose,D-Galactose,D-Mannose,L-Rhamnose and other-threonine,L-Threonine,L-Serine,Glycine and other amino acids and Fumaric acid,Acetic acid and other organic acids by promoting the metabolic pathways of Glyoxylate and dicarboxylate metabolism,Galactose metabolism and amino acid metabolism in the roots of A.senticosus.L2 treatment can significantly promote the accumulation of organic acids such as malic acid and D-gluconic acid but significantly inhibit the accumulation of carbohydrates.L1 and L4 treatments have a low promotion effect on material accumulation and significantly inhibit the accumulation of some primary metabolites.In this study,the changes of physiological characteristics,primary metabolites and medicinal active substances of A.senticosus under different UV-A supplemental light treatments were analyzed through physiological metabolism experiments.These results will help to deepen the understanding of A.senticosus and provide a theoretical basis for improving the development and utilization of A.senticosus resources.