Study On The Relationship Between The Polymorphism Of MTAP Gene Locus Rs6475592(C>T) With Coronary Heart Disease

Objective:To investigate the relationship between single nucleotide polymorphism(SNP)of methylthioadenosine phosphorylase(MTAP)gene rs6475592(C>T)with coronary heart disease(CHD),and to provide a reference for clinical prevention and treatment.Methods:A total of 192 CHD patients admitted to the Department of Cardiology of Yanbian University Hospital from 2017 to 2019 were selected as CHD group;another 192 healthy persons excluding CHD from our physical examination center during the same period were selected as the control group.All subjects’basic and clinical data were obtained and all study subjects were from Yanbian Prefecture.Collected 2ml venous blood and extracted whole blood DNA.Genotyping tests were performed using Mass ARRAY technology.SPSS 25.0 software was used for statistical analysis,and Pearsonχ~2test was used for Hardy-Weinberg Equilibrium(HWE)to ensure population representation of the subjects.Quantitative data were analyzed by independent sample t-test;qualitative data were analyzed byχ~2test.The association between MTAP rs6475592 polymorphism and CHD was analyzed by unconditional logistic regression.P<0.05 was considered as statistically significant difference.Result:(1)7 of the 384 study subjects had undetected genotypes(5 in the CHD group and 2 in the control group),and 377 study subjects were finally included for statistical analysis(187 in the CHD group and 190 in the control group).(2)Genotype distributions between CHD and control groups were in accordance with Hardy Weinberg equilibrium,and were representative of the population(both P>0.05).(3)There was no significant difference in allele frequency distribution between CHD group and control group(P=0.486),but there was significant difference in genotype distribution(P=0.004).(4)In the co-dominant model,individuals carrying the TC genotype had a lower risk of CHD compared with individuals carrying the CC genotype(adjusted OR=0.523,95%CI 0.309-0.888,P=0.016),whereas individuals carrying the TT genotype had an increased risk of CHD(adjusted OR=2.786,95%CI 1.362-5.699,P=0.005);in the recessive model,individuals carrying the TT genotype had an increased risk of CHD compared to those carrying the CC+TC genotype(adjusted OR=3.796,95%CI 1.993-7.231,P<0.001).(5)In the Korean population,in the co-dominant model,individuals carrying the TC genotype had a lower risk of CHD compared with individuals carrying the CC genotype(adjusted OR=0.375,95%CI 0.179-0.786,P=0.009),whereas individuals carrying the TT genotype had an increased risk of CHD(adjusted OR=4.990,95%CI1.779-13.993,P=0.002);in the recessive model,individuals carrying the TT genotype had an increased risk of CHD compared to those carrying the CC+TC genotype(adjusted OR=8.471,95%CI 3.228-22.235,P<0.001).In contrast,in the Han Chinese population,the differences between the two groups were not statistically significant under the alleles and various models(all P>0.05).Conclusion:MTAP gene rs6475592(C>T)locus polymorphism may be associated with CHD.TC genotype is a protective factor for CHD,TT genotype is a risk factor for CHD.