| Objective:Through the bioinformatics analysis of various components in the tumor micro-environment(TME)of malignant melanoma(MM),the biomarkers that may affect the tumor micro-environment remodeling of MM were found,and the differentially mutated genes and differentially expressed genes in MM patients were analyzed,and key genes were screened out from them,and the key genes were explored to explore the impact of key genes on the occurrence,development,and development of malignant melanoma.Whether metastasis and patient survival rate have an impact,it is hoped that the discovery of key genes will provide new therapeutic insights for cutaneous malignant melanoma.Methods: Downloading RNA-seq and somatic mutation data from the TCGA database of 472 melanoma patients was the method employed.Firstly,the ESTIMATE calculation method was used to score the immune components and matrix components of each malignant melanoma patient,and the patients were divided into high immunity and low immunity according to the scores,and the survival data of different high and low groups patients were analyzed by using the survival software package and ggpubr software package in R software,and the correlation analysis was carried out in combination with the clinical information of patients.Differential analysis of transcriptome gene data of different groups to identify differentially expressed genes,functional annotation and pathway enrichment analysis of differentially expressed genes based on the Gene Ontology GO database and the Kyoto Encyclopedia of Genes and Genomes KEGG database to determine which of the differentially expressed genes are closely related to the biological behavior of tumors.Subsequently,the differentially expressed gene and the differential mutant gene were intersected to obtain the immune-related target genes PSMB8,FAM216 B,DYSF,FAM131 C,and finally selected PSMB8 as an immunology-related prognostic marker by querying the Gene Cards gene information database and literature retrieval,and we used the Cibersort method to further confirm the relationship between PSMB8 expression and immune components.The relationship between tumor-infiltrating cells and PSMB8 gene expression was analyzed.Results:In the tumor micro-environment of MM patients,immune components have more important significance for their tumor invasion and metastasis,through the screening of differentially expressed genes in different immune groups,the key gene PSMB8 was finally selected,the high expression of this gene was positively correlated with the overall survival of patients,so as to infer that PSMB8 may be a protective factor for MM patients,further Ciber Sort analysis showed that it was positively correlated with the content of a variety of immune cells,and confirmed the effect of PSMB8 on TME from a certain angle.Especially the effect on immune components.In addition,the expression of common immune Checkpoints ICPs was higher in the group with high PSMB8 expression,suggesting that PSMB8 may have the potential to predict MM immunotherapy response.Conclusion:1.Through comprehensive bioinformatics analysis,we concluded that in the tumor microenvironment of malignant melanoma,immune components are important for tumor invasion,metastasis and patient survival.2.There were differences in the expression of PSMB8 in patients with high and low immune groups,and there was a positive correlation with the content of a variety of immunoinfiltrating cells.3.According to the close relationship between PSMB8 expression and overall survival rate of MM patients,MM tumor stage,content of immune components in TME,and detection of common ICPs,we can determine that PSMB8 is an indicator of reshaping TME status,and also has the potential to be used as an effective immunotherapy and predictor of clinical prognosis,providing a direction for us to find biological markers of MM and new therapeutic targets. |
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