| Objective: 1.To study the changes of limb motor function and middle cerebral artery cerebral blood flow(MCAO)in rats with middle cerebral artery occlusion(MCAO)treated with electroacupuncture(EA)preconditioning at Fengchi(GB20)point by Catwalk gait technique,laser Doppler cerebral blood flow monitoring technique and histomorphology.2.To observe the effect of electroacupuncture pretreatment at Fengchi point on the expression of Cofilin-1 and Profilin-2 protein in rat brain tissue.Methods: Thirty-six male SPF SD rats were randomly divided into control group,model group and electroacupuncture group.The control group did not receive any treatment,the model group underwent coil method to prepare MCAO model,the electroacupuncture group underwent coil method to prepare MCAO model and electroacupuncture pretreatment at fengchi(GB20)point.Each group was performed at each time point.1.Bederson modified neurological deficit score;2.Cat Walk gait system;3.body weight measurement;4.Laser Doppler cerebral blood flow monitoring;5.TTC staining and Western blot index detection.Results:1.The results of body weight showed that the body weight of rats in the control group continued to increase during the observation time point,and the body weight was higher than that of rats in the model group(P < 0.05);24 hours after the model,the body weight of rats in the model electroacupuncture group began to decrease,and the rate of decrease of rats in the model group was greater than that of rats in the electroacupuncture group(P < 0.05).2.Bederson modified neurological severity score showed that 24 hours after MCAO model,compared with the control group,MCAO model(electroacupuncture group,model group)showed different degrees of nerve defect symptoms(P < 0.05);on the 3rd day after model,the nerve defect score of model electroacupuncture group decreased,and the Bederson modified neurological severity score of model group was higher than that of electroacupuncture group(P < 0.05),and also higher than that of control group(P < 0.05).On the 5th day after modeling and the 7th day after modeling,the nerve defect score of the model electroacupuncture group continued to decrease,and the score of the model group was higher than that of the control group(P < 0.05).3.Cat Walk gait results:(1)Run average speed and Run duration: After MCAO model,the movement speed of rats in the model group and electroacupuncture group was significantly slowed down and the movement duration was increased compared with the control group(P < 0.05).On the 3rd day after model establishment,the movement speed of rats in the model control group began to slow down and the duration began to increase(P < 0.05).Compared with the electroacupuncture model group,the duration decreased and the movement speed increased(P < 0.05).(2)Stand: After MCAO model,the standing time of limbs increased in both model group and electroacupuncture control group(P <0.05).On the 3rd day after model establishment,the standing time of rats in the model electroacupuncture group began to decrease,and the standing time began to prolong compared with the model control group(P < 0.05).Compared with the electroacupuncture control group,the standing time of the other three limbs was significantly reduced except the standing time of the left hind limb(P < 0.05).(3)Swing speed: Compared with the control group,the limb swing velocity of rats in the electroacupuncture hindlimb model group slowed down after 24 hours,and the difference in the left limb swing velocity was statistically significant(P < 0.05).On the 3rd day after the model,the swing speed of the rats in the electroacupuncture group began to increase,the swing speed of the rats in the model group slowed down compared with the control group(P < 0.05),and the swing speed of the left hind limb increased compared with the electroacupuncture control group(P < 0.05).On the 5th day after model establishment,the limb swing speed of MCAO model electroacupuncture group continued to increase,and there was a statistically significant difference in the swing speed between the right hind limb and the left hind limb compared with the model control group(P < 0.05),and there was a statistically significant difference in the swing speed between the electroacupuncture model group and the left hind limb(P < 0.05).On the 7th day after model establishment,the limb swing speed of rats in each group continued to increase.Compared with the model control group,there was no significant difference in the swing speed of the other three sides of the body in each group except the swing speed of the left hind limb(P > 0.05).(4)Stride length: After 24 hours of MCAO model,the body velocity of the extremities of the rats became slower than that of the control group(P < 0.05).On the 3rd day after model establishment,the body velocity of the extremities began to increase in the model electroacupuncture group,and the body velocity slowed down compared with the model control group(P < 0.05),and the body velocity of the extremities recovered faster compared with the electroacupuncture model group(P < 0.05).On the 5th day after modeling and the 7th day after modeling,the body velocity of the left forelimb and left hind limb was slowed down in the model group compared with the control group(P < 0.05),and the body velocity of the left hind limb was increased in the electroacupuncture model group compared with the control group(P < 0.05).4.Laser Doppler monitoring showed that after MCAO model,the cerebral blood flow of rats in model electroacupuncture group was less than that in control group(P <0.05).At the time of reperfusion,the cerebral blood flow of the model electroacupuncture group gradually increased,the cerebral blood flow of the model group was less than that of the control group(P < 0.05),and the cerebral blood flow recovery was better in the electroacupuncture model group than in the control group(P < 0.05).5.TTC staining showed that TTC staining was dark red in normal brain tissue and white in ischemic infarct tissue.Compared with the control group,the cerebral infarction area of rats in the model group was significantly larger(P < 0.05);compared with the model group,the cerebral infarction area of rats in the electroacupuncture group was significantly smaller(P < 0.05).6.Western blot results: The results of Profilin-2 protein showed that compared with the control group,the expression of Profilin-2 protein in the model group was less(P < 0.05),and compared with the model group,the expression of Profilin-2 protein in the electroacupuncture group was more(P < 0.05);the results of Cofilin-1 protein showed that compared with the control group,the expression of Cofilin-1 protein in the model group was less(P > 0.05),and compared with the model group,the expression of Cofilin-1 protein in the electroacupuncture group was more(P > 0.05).Conclusion:1.Electroacupuncture preconditioning promotes the improvement of motor function of left hind limb in rats after MCAO,and can also improve the function of unaffected limb and restore the coordination and stability of motor function in rats;2.Electroacupuncture preconditioning improves the motor function injury caused by cerebral ischemia-reperfusion injury in the post-model recovery stage of rats and can promote the better recovery of motor function of MCAO rats.3.pre-intervention at Fengchi point has a neuroprotective effect on MCAO rats,and the possible mechanism is to exert a neuroprotective effect by up-regulating Cofilin-1 and Profilin-2 expression and improve limb motor function in MCAO rats. |
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