| Preface: Compared to decades ago,due to the boom of the Internet,children today spend more time on electronic products and lead a sedentary lifestyle,which leads them to overlook the importance of participating in physical activities during childhood and adolescence.Studies have shown that active physical activity in children and adolescents improves learning and memory,and animal models have demonstrated that physical activity improves cognitive function in developing mice.Developmental period is an important and sensitive period of cognitive development.However,studies on the relationship between exercise and cognitive function in developmental period are limited.The neural mechanism of the influence of voluntary exercise on synaptic transmission,learning and memory in the developmental brain is still unclear.Methods: Developing mice underwent voluntary wheel running for 4 weeks starting from 30 days of age.To explore the effect of voluntary exercise on basal inhibitory synaptic transmission in hippocampus of developing mice.The amount of exercise of mice within 30 days was recorded,and the micro-inhibitory postsynaptic current(m IPSCs)and α1gamma-amine-butyric acid(GABA)in the hippocampus of mice were detected by patch clamp technique and immunofluorescence technique.Results: The frequency of micro-inhibitory postsynaptic currents(m IPSCs)in male and female DG neurons also increased significantly,but the amplitude of m IPSCs in DG neurons did not change significantly.The amplitude of m IPSCs increased significantly in male CA3 neurons without significant changes in frequency,whereas the frequency of m IPSCs increased significantly in female CA3 neurons without significant changes in amplitude.At the same time,m IPSCs in hippocampal CA1 neurons of male and female mice were not affected by voluntary wheel running during development.In addition,the expression of inhibitory gamma-aminobutyric acid GABAA subunit in DG and CA3 was significantly increased,while no change was observed in CA1.Conclusions: These results suggest that autonomous wheel running can enhance synaptic transmission by increasing the expression of α1 GABA receptors in the hippocampus DG and CA3 and the frequency and amplitude of m IPSCs,and ultimately promote the development of learning and memory in mice.The purpose of these studies is to understand the neural mechanisms underlying the effects of voluntary exercise on learning and memory in mice.This study aims to supplement the neural mechanism of voluntary exercise on learning and memory in mice,and provide reference for children participating in sports activities to contribute to intellectual development. |
