Studies On Germplasm Resources Of Auricularia Polytricha

Auricularia polytricha (Coarse moer) is a kind of traditional Chinese medicinal fungi, as well as edible fungi, which belongs to Auricula, Auriculariaceae, Auriculariales, Heterobasidiomycetes, Basidiomycotina, Eumycota. It is one of the main cultivated edible mushroom in China. Its strains for cultivation are of characteristics with thick , soft ear slice and rich nutrition. It tastes delicious, fresh and crisp. It is calm and sweet when used as medicine with function of breath clear, blood activating etc.. As ecological environment gradually become deteriorated, the wild growing coarse moer are less and less. The cultivation is in chaos, which makes the varieties degeneration worse, disease and insect pest quite widespread. So, the collection and conservation of germplasm resources for A. polytricha, also strengthening research on germplasm resources of A. polytricha is an essential task. This study evaluated the germplasm resources of A. polytricha on the level of isozyme and molecular level. In the meantime, the productivity of strains was studied. Furthermore, the preliminary research was done on the optimal condition of Cu absorption by .A. polytricha.1. Esterase isozyme zymogram polymorphisms of 56 strains in A. polytricha were investigated using vertical slab polyacrylamide gel electrophoresis. The results showed that there existed diversity among coarse muer strains. Seventy-six zymogram bands were observed and there were 26 zymogram types in all tested strains. Each strain had 1-6 bands respectively. Differences of strains were expressed in their respective characteristic zymograms in the study. The results of cluster analysis at 67% similarity level showed that all strains tested could be clustered into 8 groups, i.e. the first group including 34 strains of huang-er zh etc., the second group including 21 strains of white back moer etc., the third and the forth group only single strain of A. auricla and 915 respectively, the fifth group including 4 strains of 99 etc., the sixth group including 4 strains of 43 etc., the seventh group including 2 strains of 50385 and APO67, the eighth group including 2 strains of xiao-shang 3 and hybrid 34. The analytical technique of esterase isozyme was an available method for identifying strains in species and under species.2. The random amplified polymorphic DNA (RAPD) analysis was conducted with 22 ten-nucleotide random primers in 56 strains of A. polytricha collected from various...