The Study On Syndrome Of Children's Virus Pneumonia And The Effect Of Dispersing Lung To Resolve Phlegm On Fas Expression

ObjectiveClinical part: To probe into the syndrome characteristics of virus pneumonia of children by clinical observations, and summarize the regularity of syndrome differentiation.Experiment Part: To determine the effects of adenovirus (adv) 31 and 7b on the expression of Fas. To evaluate the effects of Qing Fei oral liquids on the expression of Fas in human embryonic lung fibroblast cells which were infected by adenovirus 31 and 7b.MethodsClinical part: Observed the syndrome characteristics of virus pneumonia of children in Nanjing, Tianjin and Changsha. Filled in the case report form. Then, analysed the clinical data.Experiment Part: Attacted human embryonic lung fibroblast cells with adenovirus 31 and 7b, then added the serum with Qing Fei oral liquid orRiba Vivir (RBV) in different groups. Investigated the expression of Fas protein in different groups by flow cytometric cell cycle analysis.ResultsClinical part: There were significant differences in fever, sputum, short breath, flaring of nares, auscultation on lung, aversion to cold, complexion, vitality, sweating, tonsil, finger loops and pulse, fur and texture of tongueamong the five syndromes. There were no significant differences in cough, nasal mucus, appetite, thirst, nausea, feces, urine, positive rate and types of virus among the five syndromes. We induced discriminant function for syndrome differentiation of children's virus pneumonia. Wind and cold invasion of lung: Y=-17.237+0.560fever+1.003tonsil-3.210factorr5.230factor2- 1.721 factor3-0.475factor4+ 1 .332factor5+ 1 .603factor6-0.602factory- 1 .007factor8 Wind and heat invasion of lung: Y=-4.830+1 .054fever+0.858tonsil-2. 183 factor1 -0. 837factor2-0. 127factor3+0.173factor4 +0.740factor5+0.381factor6-0.198factory +0.495factor8 Accumulation of phlegm-heat in lung: Y=-3.525+0.905fever+l .015tonsil-0.472factor1+0.3 1 Ifactor2+0.48 1 factor3-0.088factor 4+0.0 1 6factor5-0.230factor6+0.053factor7+0.221 factor8 Asthenia of pulmonosplenic qi: Y=-5. 048+0. 192fever +0.685tonsil +0.524factor 1+2.719factor2-0.7 14factor3+0.344factor4+ 1 .054factor5+0.636factor6+0.07 1factor7+0.541 factor8 Yin asthenia causing pulmonary heat:Y=-9.483+0. 128fever+0.396tonsil+1.513factor1+4.562factor2-0.798factor3+ 1.659factor4+1 . 1 95factor5-0.446factor6+0.037factor7-0.289factor8Experiment Part: The expression of Fas protein of adenovirus-infected group was downregulated compared with the nomal control. The expression of Fas protein of Qing Fei oral liquid group was upregulated compared with the adenovirus-infected control. There was no significant difference between the Qing Fei oral liquid group and RBV group.ConclusionClinical part: Different symptoms have different values in syndrome differentiation. We can distinguish the syndrome by referring to the function.Experiment Part: Adenovirus 3I and 7b have influence on the expression of Fas protein. Qing Fei oral liquid can upregulate the expression of Fas protein which was effected by Adenovirus 3I and 7b.