The Intervention Effect Of Supplementing Qi, Activating Blood Circulation, Resolving Phlegm And Its Different Combination Regimens On COPD Rats

Objective:To observe the intervention effect of Supplementing Qi, activating blood circulation, resolving phlegm and its combination regimens on airway inflammation and airway remodeling of COPD rats from the aspect of cytology, molecular biology and pathology. Through comparing and evaluating the efficacy of different treatment methods, we explore its possible mechanism action, and provide effective and reliable experimental basis for TCM on COPD.Method:90SD male rats were randomly divided into nine groups, meantime COPD rat model was established by intratracheal instillation of LPS and exposure to cigarette smoking daily. Specific subgroups and delivery are as follows:①Supplementing Qi group:Administrated by decoction of supplementing Qi②Activating blood circulation group:Administrated by decoction of activating blood circulation③Resolving phlegm group:Administrated by decoction of resolving phlegm④Supplementing Qi and activating blood circulation group:Administrated by decoction of Supplementing Qi and activating blood circulation⑤Supplementing Qi and resolving phlegm group:Administrated by decoction of Supplementing Qi and resolving phlegm⑥Activating blood circulation and resolving phlegm group:Administrated by decoction of activating blood circulation and resolving phlegm⑦Supplementing Qi, activating blood circulation and resolving phlegm group: Administrated by decoction of Supplementing Qi, activating blood circulation and resolving phlegm⑧The model group:Administrated by physiological saline⑨The normal group:Administrated by physiological salineEach group was administrated from the fourth day of molding, once a day, medication for30days.Using cell counting and HE staining method to measure white blood cell count and the percentage of neutrophil, macrophage and lymphocyte in BALF, ELISA method to measure the content of cytokine IL-8and TNF-α in BALF, immunohistochemical method to measure the protein expression of u-PA, TGF-β1and Elastin in lung tissue, to observe the pulmonary tissue structural changes under the microscope, and measure the thickness of bronchial duct wall and smooth muscle with image analyzer.Result:1. The changes of inflammatory cells and cytokines in BALFThere was significant difference in different groups in white blood cell count,the percentage of neutrophil, macrophage and lymphocyte, and the content of cytokine IL-8and TNF-α in BALF (F=121.04, P=0.000<0.05; F=64.97, P=0.000<0.05; F=77.83, P=0.000<0.05; F=23.4, P=0.000<0.05; F=17.2, P=0.000<0.05). There was no significant difference in different groups in lymphocyte (F=0.74, P=0.653>0.05). The comparison of each two groups in white blood cell count,the percentage of neutrophil, macrophage and lymphocyte, and the content of cytokine IL-8and TNF-α in BALF show:the model group comparison with control group, supplementing Qi, activating blood circulation and resolving phlegm group, activating blood circulation and resolving phlegm group, supplementing Qi and resolving phlegm group, supplementing Qi and activating blood circulation group, activating blood circulation group; the control group comparison with supplementing Qi, activating blood circulation and resolving phlegm group, activating blood circulation and resolving phlegm group, supplementing Qi and resolving phlegm group, supplementing Qi and activating blood circulation group, activating blood circulation group, resolving phlegm group, and supplementing Qi group; supplementing Qi, activating blood circulation and resolving phlegm group comparison with activating blood circulation group, resolving phlegm group, supplementing Qi group, and supplementing Qi and resolving phlegm group; supplementing Qi and activating blood circulation group,activating blood circulation and resolving phlegm group comparison with activating blood circulation group, resolving phlegm group, supplementing Qi group; supplementing Qi and resolving phlegm group and activating blood circulation group comparison with resolving phlegm group and supplementing Qi group, there was significant difference (P<0.05). But comparison among supplementing Qi, activating blood circulation and resolving phlegm group, activating blood circulation and resolving phlegm group, and supplementing Qi and activating blood circulation group; supplementing Qi and resolving phlegm group and activating blood; the model group, resolving phlegm group, and supplementing Qi group was no significant difference (P>0.05).2. The thickness changes of bronchial duct wall and smooth muscleThe morphologic changes of lung:the control group was normal, while the model group was showed significant pathological changes in the lung. While the other group except for resolving phlegm group, and supplementing Qi group, was improved in different level. The level of improvement from high to low was supplementing Qi, activating blood circulation and resolving phlegm group, activating blood circulation and resolving phlegm group, supplementing Qi and resolving phlegm group, supplementing Qi and activating blood circulation group, activating blood circulation group.The statistical results show:There was significant difference in different groups in the thickness of bronchial duct wall and smooth muscle (F=133.6, P=0.000<0.05; F=65.6, P=0.000<0.05). The comparison of each two groups in the thickness of bronchial duct wall and smooth muscle show:the model group, supplementing Qi group and resolving phlegm group comparison with control group, supplementing Qi, activating blood circulation and resolving phlegm group, activating blood circulation and resolving phlegm group, supplementing Qi and resolving phlegm group, supplementing Qi and activating blood circulation group, activating blood circulation group; the control group comparison with supplementing Qi, activating blood circulation and resolving phlegm group, activating blood circulation and resolving phlegm group, supplementing Qi and resolving phlegm group, supplementing Qi and activating blood circulation group, activating blood circulation group; activating blood circulation group comparison with supplementing Qi and activating blood circulation group, activating blood circulation and resolving phlegm group, supplementing Qi, activating blood circulation and resolving phlegm group; supplementing Qi and resolving phlegm group comparison with supplementing Qi, activating blood circulation and resolving phlegm group, there was significant difference (P<0.05). But comparison among supplementing Qi, activating blood circulation and resolving phlegm group, activating blood circulation and resolving phlegm group, and supplementing Qi and activating blood circulation group; supplementing Qi and resolving phlegm group and activating blood; the model group, resolving phlegm group, and supplementing Qi group was no significant difference (P>0.05).3. The changes of protein expression of u-PA, TGF-β1and Elastin in lung tissue The statistical results show:There was significant difference in different groups in the protein expression of u-PA, TGF-β1and Elastin of lung tissue (P=0.000<0.05). The comparison of each two groups in the protein expression of u-PA, TGF-β1and Elastin of lung tissue show:the model group, supplementing Qi group and resolving phlegm group comparison with control group, supplementing Qi, activating blood circulation and resolving phlegm group, activating blood circulation and resolving phlegm group, supplementing Qi and resolving phlegm group, supplementing Qi and activating blood circulation group, activating blood circulation group; supplementing Qi and resolving phlegm group and activating blood circulation group comparison with the control group; activating blood circulation group comparison with supplementing Qi and activating blood circulation group, activating blood circulation and resolving phlegm group, supplementing Qi, activating blood circulation and resolving phlegm group; supplementing Qi and resolving phlegm group comparison with supplementing Qi, activating blood circulation and resolving phlegm group, there was significant difference (P<0.05). But comparison among supplementing Qi, activating blood circulation and resolving phlegm group, activating blood circulation and resolving phlegm group, and supplementing Qi and activating blood circulation group; supplementing Qi and resolving phlegm group and activating blood; the model group, resolving phlegm group, and supplementing Qi group was no significant difference (P>0.05).Conclusion:1. The management of supplementing Qi, activating blood circulation and resolving phlegm group, activating blood circulation and resolving phlegm, Supplementing Qi and resolving phlegm, Supplementing Qi and activating blood circulation, activating blood circulation can inhibit accumulation, increase and activation of neutrophil and macrophage, reduce secretion of cytokine IL-8and TNF-α of BALF, and then relieve inflammation of COPD rat.2. The management of supplementing Qi, activating blood circulation and resolving phlegm group, activating blood circulation and resolving phlegm, Supplementing Qi and resolving phlegm, Supplementing Qi and activating blood circulation, activating blood circulation can inhibit the expression of u-PA and TGF-β1,meanwhile enhance the expression of elastin in COPD rat lung tissue.3. The management of supplementing Qi, activating blood circulation and resolving phlegm group, activating blood circulation and resolving phlegm, Supplementing Qi and resolving phlegm, Supplementing Qi and activating blood circulation, activating blood circulation can reduce thickening trend of bronchial duct wall and smooth muscle. 4. Tthe management of supplementing Qi, activating blood circulation and resolving phlegm group, activating blood circulation and resolving phlegm, Supplementing Qi and resolving phlegm, Supplementing Qi and activating blood circulation, activating blood circulation can intervent and delay COPD airway remodeling process, which maybe through the regulation of the expression of u-PA, TGF-β1and elastin in COPD rat lung tissue.5. Activating blood circulation group was effective comparing with resolving phlegm group and supplementing Qi group.6. The effect of supplementing Qi, activating blood circulation and resolving phlegm group, activating blood circulation and resolving phlegm group, supplementing Qi and activating blood circulation group is better than supplementing Qi and resolving phlegm group and activating blood circulation group.