Ischemic Stroke And E-selectin And Icam-1 And Cx3cr1 Gene Polymorphisms Related Research

Objective To study the relationship between polymorphisms of E-selectin gene,fractalkine receptor CX3CR1,intercellular adhesion molecule-1(ICAM-1) gene and Cerbral Thrombosis in Han people of Liaoning province and searched the genetic markers of Cerbral Thrombosis.Methods The A561C polymorphism in the exon 4 of E-selectin gene,ICAM-1and CX3CR1 were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)analysis and DNA sequencing among 360 patients with Cerbral Thrombosis of Han people in Liaoning and 306 healthy controls.Results 1. There was significant difference in frequencies of allele and genotype in A561C polymorphism between Cerbral Thrombosis and control groups respectively (X2=6.39,P<0.05).The relative risk suffered from Cerbral Thrombosis of AC genotype was 3.05 times of the AA genotype(OR=3.05,95%CI:1.29-7.20). Allele frequency in CC and AC is also significant difference(X2 =5.94 P < 0.05,OR = 2.84,95% CI:1.23-6.55) ,and the serum E-selectin level was significantly higher among carriers of AC genotype as compared with non-carriers (AA genotype) [(58.62±14.47)ng/mlVS(45.28±13.86)ng/ml,P<0.01].2. The EE genotype of ICAM-1 in the cerebral thrombosis group are higher than control group(X2=8.03,P<0.01).The allele gene E/K have statistical significance in two group(X2=11.01,P<0.01).3. There was significant difference in both polymorphisms between patients with Cerbral Thrombosis and controls,the adjusted odds ratio respectively was 0.51(95%CI: 0.27-0.95, P<0.05) and 0.48 (95%CI: 0.28-083, P<0.05). There was significant difference in frequencies of allele I249 between Cerbral Thrombosis and control groups . The adjusted odds ratio was 0.54(95% CI:0.35 -0.84, P < 0.05). CX3CR1 I249 was associated with a markedly reduced risk of Cerbral Thrombosis.Conclusion 1. E-se1ectin A561C polymorphism was associated with Cerbral Thrombosis, and C allele may be a genetic risk factor of Cerbral Thrombosis among Han people,in whom the E-selectin AC genotype carriers maybe increase the risk of Cerbral Thrombosis by enhancing the E-selectin expression.2. ICAM-1E/K polymorphism was associated with Cerbral Thrombosis, and E allele may be a genetic risk factor of Cerbral Thrombosis among Han people,but C allele may have an effect of the protection.3. The results show that CX3CR1 I249 is an independent genetic risk factor for Cerbral Thrombosis and that CX3CR1 may be involved in the pathogenesis of athero sclerotic disease.